Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

Original languageEnglish
Pages (from-to)303-312
Number of pages10
JournalBioresource Technology
Volume280
DOIs
Publication statusPublished - 2019
MoE publication typeNot Eligible

Fingerprint

Enzyme inhibition
Enzymatic hydrolysis
Lignin
lignin
hydrolysis
Cellulose
Hydrolysis
Straw
straw
wheat
Cellulose 1,4-beta-Cellobiosidase
effect
Cellulase
structural change
Biomass
adsorption
Adsorption
biomass

Keywords

  • Cellulase
  • Non-productive binding
  • Saccharification
  • Spruce
  • Wheat straw

Cite this

@article{3506176882564f52bc9b8d3ff35c7313,
title = "Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis",
abstract = "Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.",
keywords = "Cellulase, Non-productive binding, Saccharification, Spruce, Wheat straw",
author = "Miriam Kellock and Hannu Maaheimo and Kaisa Marjamaa and Jenni Rahikainen and Heng Zhang and Ulla Holopainen-Mantila and John Ralph and Tarja Tamminen and Claus Felby and Kristiina Kruus",
year = "2019",
doi = "10.1016/j.biortech.2019.02.051",
language = "English",
volume = "280",
pages = "303--312",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis. / Kellock, Miriam; Maaheimo, Hannu; Marjamaa, Kaisa; Rahikainen, Jenni; Zhang, Heng; Holopainen-Mantila, Ulla; Ralph, John; Tamminen, Tarja; Felby, Claus; Kruus, Kristiina.

In: Bioresource Technology, Vol. 280, 2019, p. 303-312.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

AU - Kellock, Miriam

AU - Maaheimo, Hannu

AU - Marjamaa, Kaisa

AU - Rahikainen, Jenni

AU - Zhang, Heng

AU - Holopainen-Mantila, Ulla

AU - Ralph, John

AU - Tamminen, Tarja

AU - Felby, Claus

AU - Kruus, Kristiina

PY - 2019

Y1 - 2019

N2 - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

AB - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

KW - Cellulase

KW - Non-productive binding

KW - Saccharification

KW - Spruce

KW - Wheat straw

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U2 - 10.1016/j.biortech.2019.02.051

DO - 10.1016/j.biortech.2019.02.051

M3 - Article

VL - 280

SP - 303

EP - 312

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -