Abstract
Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.
| Original language | English |
|---|---|
| Pages (from-to) | 303-312 |
| Number of pages | 10 |
| Journal | Bioresource Technology |
| Volume | 280 |
| DOIs | |
| Publication status | Published - 2019 |
| MoE publication type | Not Eligible |
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Keywords
- Cellulase
- Non-productive binding
- Saccharification
- Spruce
- Wheat straw
Cite this
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Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis. / Kellock, Miriam; Maaheimo, Hannu; Marjamaa, Kaisa; Rahikainen, Jenni; Zhang, Heng; Holopainen-Mantila, Ulla; Ralph, John; Tamminen, Tarja; Felby, Claus; Kruus, Kristiina.
In: Bioresource Technology, Vol. 280, 2019, p. 303-312.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis
AU - Kellock, Miriam
AU - Maaheimo, Hannu
AU - Marjamaa, Kaisa
AU - Rahikainen, Jenni
AU - Zhang, Heng
AU - Holopainen-Mantila, Ulla
AU - Ralph, John
AU - Tamminen, Tarja
AU - Felby, Claus
AU - Kruus, Kristiina
PY - 2019
Y1 - 2019
N2 - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.
AB - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.
KW - Cellulase
KW - Non-productive binding
KW - Saccharification
KW - Spruce
KW - Wheat straw
UR - http://www.scopus.com/inward/record.url?scp=85061573022&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2019.02.051
DO - 10.1016/j.biortech.2019.02.051
M3 - Article
AN - SCOPUS:85061573022
VL - 280
SP - 303
EP - 312
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -