Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Miriam Kellock; Hannu Maaheimo; Kaisa Marjamaa; Jenni Rahikainen; Heng Zhang; Ulla Holopainen-Mantila; John Ralph; Tarja Tamminen; Claus Felby; Kristiina Kruus

doi:10.1016/j.biortech.2019.02.051

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Miriam Kellock, Hannu Maaheimo, Kaisa Marjamaa, Jenni Rahikainen, Heng Zhang, Ulla Holopainen-Mantila, John Ralph, Tarja Tamminen, Claus Felby, Kristiina Kruus^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

94 Citations (Scopus)

Abstract

Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

Original language	English
Pages (from-to)	303-312
Number of pages	10
Journal	Bioresource Technology
Volume	280
DOIs	https://doi.org/10.1016/j.biortech.2019.02.051
Publication status	Published - 2019
MoE publication type	Not Eligible

Funding

This work was supported by the Fortum Foundation , Espoo, Finland. J. Ralph was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-SC0018409). Kirsi Kiiveri is acknowledged for technical assistance in enzyme activity experiments and Liisa Änäkäinen is thanked for taking the fluorescence microscope images. Appendix A

Keywords

Cellulase
Non-productive binding
Saccharification
Spruce
Wheat straw

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.02.051

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title = "Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis",

abstract = "Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.",

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author = "Miriam Kellock and Hannu Maaheimo and Kaisa Marjamaa and Jenni Rahikainen and Heng Zhang and Ulla Holopainen-Mantila and John Ralph and Tarja Tamminen and Claus Felby and Kristiina Kruus",

note = "Funding Information: This work was supported by the Fortum Foundation , Espoo, Finland. J. Ralph was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-SC0018409). Kirsi Kiiveri is acknowledged for technical assistance in enzyme activity experiments and Liisa {\"A}n{\"a}k{\"a}inen is thanked for taking the fluorescence microscope images. Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2019",

doi = "10.1016/j.biortech.2019.02.051",

language = "English",

volume = "280",

pages = "303--312",

journal = "Bioresource Technology",

issn = "0960-8524",

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AU - Kellock, Miriam

AU - Maaheimo, Hannu

AU - Marjamaa, Kaisa

AU - Rahikainen, Jenni

AU - Zhang, Heng

AU - Holopainen-Mantila, Ulla

AU - Ralph, John

AU - Tamminen, Tarja

AU - Felby, Claus

AU - Kruus, Kristiina

N1 - Funding Information: This work was supported by the Fortum Foundation , Espoo, Finland. J. Ralph was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-SC0018409). Kirsi Kiiveri is acknowledged for technical assistance in enzyme activity experiments and Liisa Änäkäinen is thanked for taking the fluorescence microscope images. Appendix A Publisher Copyright: © 2019 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019

Y1 - 2019

N2 - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

AB - Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

KW - Cellulase

KW - Non-productive binding

KW - Saccharification

KW - Spruce

KW - Wheat straw

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DO - 10.1016/j.biortech.2019.02.051

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SN - 0960-8524

VL - 280

SP - 303

EP - 312

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Abstract

Funding

Keywords

UN SDGs

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