Abstract
Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.
Original language | English |
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Pages (from-to) | 303-312 |
Number of pages | 10 |
Journal | Bioresource Technology |
Volume | 280 |
DOIs | |
Publication status | Published - 2019 |
MoE publication type | Not Eligible |
Funding
This work was supported by the Fortum Foundation , Espoo, Finland. J. Ralph was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-SC0018409). Kirsi Kiiveri is acknowledged for technical assistance in enzyme activity experiments and Liisa Änäkäinen is thanked for taking the fluorescence microscope images. Appendix A
Keywords
- Cellulase
- Non-productive binding
- Saccharification
- Spruce
- Wheat straw