Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

Original languageEnglish
Pages (from-to)303-312
Number of pages10
JournalBioresource Technology
Volume280
DOIs
Publication statusPublished - 2019
MoE publication typeNot Eligible

    Fingerprint

Keywords

  • Cellulase
  • Non-productive binding
  • Saccharification
  • Spruce
  • Wheat straw

Cite this