Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Miriam Kellock, Hannu Maaheimo, Kaisa Marjamaa, Jenni Rahikainen, Heng Zhang, Ulla Holopainen-Mantila, John Ralph, Tarja Tamminen, Claus Felby, Kristiina Kruus*

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    94 Citations (Scopus)

    Abstract

    Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

    Original languageEnglish
    Pages (from-to)303-312
    Number of pages10
    JournalBioresource Technology
    Volume280
    DOIs
    Publication statusPublished - 2019
    MoE publication typeNot Eligible

    Funding

    This work was supported by the Fortum Foundation , Espoo, Finland. J. Ralph was funded by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-SC0018409). Kirsi Kiiveri is acknowledged for technical assistance in enzyme activity experiments and Liisa Änäkäinen is thanked for taking the fluorescence microscope images. Appendix A

    Keywords

    • Cellulase
    • Non-productive binding
    • Saccharification
    • Spruce
    • Wheat straw

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