Effect of hydrothermal pretreatment severity on lignin inhibition in enzymatic hydrolysis

Miriam Kellock, Hannu Maaheimo, Kaisa Marjamaa, Jenni Rahikainen, Heng Zhang, Ulla Holopainen-Mantila, John Ralph, Tarja Tamminen, Claus Felby, Kristiina Kruus

    Research output: Contribution to journalArticleScientificpeer-review

    78 Citations (Scopus)


    Hydrothermal pretreatment is commonly used for enhancing enzymatic hydrolysis of lignocellulosics. Spruce and wheat straw were pretreated with increasing severity and lignin characteristics were analysed. The effect of enzymatically isolated lignin on the hydrolysis of Avicel and the adsorption of a cellobiohydrolase onto lignin was measured. Non-pretreated lignins had only a minor effect on Avicel hydrolysis. The structural changes in lignin accompanying hydrothermal pretreatment were associated with increased binding and inactivation of the cellulase on the lignin surface. The inhibitory effect was more pronounced in spruce than in wheat straw lignin. However, similar pretreatment severities caused similar levels of inhibition in Avicel hydrolysis for both biomass sources. The combined severity factor of the pretreatment correlated well with the inhibitory effect of lignin.

    Original languageEnglish
    Pages (from-to)303-312
    Number of pages10
    JournalBioresource Technology
    Publication statusPublished - 2019
    MoE publication typeNot Eligible


    • Cellulase
    • Non-productive binding
    • Saccharification
    • Spruce
    • Wheat straw


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