Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus

Elisabet Moyano (Corresponding Author), Katja Jouhikainen, Päivi Tammela, Javier Palazon, Rosa M. Cusido, M. Teresa Pinol, Teemu Teeri, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

99 Citations (Scopus)

Abstract

In order to increase the production of the pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed to introduce the T-DNA of the Ri plasmid together with the tobacco pmt gene under the control of CaMV 35S promoter, into the genome of Datura metel and Hyoscyamus muticus. This gene codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses the first committed step in the tropane alkaloid pathway. Hairy root cultures overexpressing the pmt gene aged faster and accumulated higher amounts of tropane alkaloids than control hairy roots. Both hyoscyamine and scopolamine production were improved in hairy root cultures of D. metel, whereas in H. muticus only hyoscyamine contents were increased by pmt gene overexpression. These roots have a high capacity to synthesize hyoscyamine, but their ability to convert it into scopolamine is very limited. The results indicate that the same biosynthetic pathway in two related plant species can be differently regulated, and overexpression of a given gene does not necessarily lead to a similar accumulation pattern of secondary metabolites.
Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalJournal of Experimental Botany
Volume54
Issue number381
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Datura metel
Hyoscyamus
Hyoscyamus muticus
Tropanes
tropane alkaloids
Hyoscyamine
gene overexpression
atropine
scopolamine
Alkaloids
putrescine N-methyltransferase
Scopolamine Hydrobromide
Genes
genes
secondary metabolites
Biosynthetic Pathways
biochemical pathways
plasmids
tobacco
promoter regions

Keywords

  • Datura metel
  • hairy root cultures
  • hyoscyamine
  • Hyoscyamus muticus
  • 35S-pmt gene
  • scopolamine
  • tropane alkaloids

Cite this

Moyano, Elisabet ; Jouhikainen, Katja ; Tammela, Päivi ; Palazon, Javier ; Cusido, Rosa M. ; Pinol, M. Teresa ; Teeri, Teemu ; Oksman-Caldentey, Kirsi-Marja. / Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. In: Journal of Experimental Botany. 2003 ; Vol. 54, No. 381. pp. 203-211.
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abstract = "In order to increase the production of the pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed to introduce the T-DNA of the Ri plasmid together with the tobacco pmt gene under the control of CaMV 35S promoter, into the genome of Datura metel and Hyoscyamus muticus. This gene codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses the first committed step in the tropane alkaloid pathway. Hairy root cultures overexpressing the pmt gene aged faster and accumulated higher amounts of tropane alkaloids than control hairy roots. Both hyoscyamine and scopolamine production were improved in hairy root cultures of D. metel, whereas in H. muticus only hyoscyamine contents were increased by pmt gene overexpression. These roots have a high capacity to synthesize hyoscyamine, but their ability to convert it into scopolamine is very limited. The results indicate that the same biosynthetic pathway in two related plant species can be differently regulated, and overexpression of a given gene does not necessarily lead to a similar accumulation pattern of secondary metabolites.",
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author = "Elisabet Moyano and Katja Jouhikainen and P{\"a}ivi Tammela and Javier Palazon and Cusido, {Rosa M.} and Pinol, {M. Teresa} and Teemu Teeri and Kirsi-Marja Oksman-Caldentey",
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Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. / Moyano, Elisabet (Corresponding Author); Jouhikainen, Katja; Tammela, Päivi; Palazon, Javier; Cusido, Rosa M.; Pinol, M. Teresa; Teeri, Teemu; Oksman-Caldentey, Kirsi-Marja.

In: Journal of Experimental Botany, Vol. 54, No. 381, 2003, p. 203-211.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus

AU - Moyano, Elisabet

AU - Jouhikainen, Katja

AU - Tammela, Päivi

AU - Palazon, Javier

AU - Cusido, Rosa M.

AU - Pinol, M. Teresa

AU - Teeri, Teemu

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2003

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N2 - In order to increase the production of the pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed to introduce the T-DNA of the Ri plasmid together with the tobacco pmt gene under the control of CaMV 35S promoter, into the genome of Datura metel and Hyoscyamus muticus. This gene codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses the first committed step in the tropane alkaloid pathway. Hairy root cultures overexpressing the pmt gene aged faster and accumulated higher amounts of tropane alkaloids than control hairy roots. Both hyoscyamine and scopolamine production were improved in hairy root cultures of D. metel, whereas in H. muticus only hyoscyamine contents were increased by pmt gene overexpression. These roots have a high capacity to synthesize hyoscyamine, but their ability to convert it into scopolamine is very limited. The results indicate that the same biosynthetic pathway in two related plant species can be differently regulated, and overexpression of a given gene does not necessarily lead to a similar accumulation pattern of secondary metabolites.

AB - In order to increase the production of the pharmaceuticals hyoscyamine and scopolamine in hairy root cultures, a binary vector system was developed to introduce the T-DNA of the Ri plasmid together with the tobacco pmt gene under the control of CaMV 35S promoter, into the genome of Datura metel and Hyoscyamus muticus. This gene codes for putrescine:SAM N-methyltransferase (PMT; EC. 2.1.1.53), which catalyses the first committed step in the tropane alkaloid pathway. Hairy root cultures overexpressing the pmt gene aged faster and accumulated higher amounts of tropane alkaloids than control hairy roots. Both hyoscyamine and scopolamine production were improved in hairy root cultures of D. metel, whereas in H. muticus only hyoscyamine contents were increased by pmt gene overexpression. These roots have a high capacity to synthesize hyoscyamine, but their ability to convert it into scopolamine is very limited. The results indicate that the same biosynthetic pathway in two related plant species can be differently regulated, and overexpression of a given gene does not necessarily lead to a similar accumulation pattern of secondary metabolites.

KW - Datura metel

KW - hairy root cultures

KW - hyoscyamine

KW - Hyoscyamus muticus

KW - 35S-pmt gene

KW - scopolamine

KW - tropane alkaloids

U2 - 10.1093/jxb/erg014

DO - 10.1093/jxb/erg014

M3 - Article

VL - 54

SP - 203

EP - 211

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

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