Effect of lactic acid bacteria inoculants, formic acid, potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage

Eeva Saarisalo, Taina Jalava, Eija Skyttä, Auli Haikara, Seija Jaakkola

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

The efficiency of a novel strain of lactic acid bacteria inoculant (Lactobacillus plantarum VTT E-78076, E76) on the fermentation quality of wilted silage was studied. Furthermore, the possibility to improve aerobic stability of silages by combining an inoculant and chemical preservatives was investigated. Two experiments were conducted with wilted timothy-meadow fescue herbage (dry matter 429 and 344 g kg-1) using six treatments. In experiment I, E76 (106 cfu g-1 fresh matter (FM)) was applied alone and in combination with sodium benzoate (0.3 g kg-1 grass FM) or low rate of formic acid (0.4 l t-1 FM). In experiment II, E76 and a commercial inoculant were applied alone and in combination with sodium benzoate. Untreated silage and formic acid (4 l t-1 FM) treated silage served as negative and positive controls in both experiments. The effect of sodium benzoate and potassium sorbate in experiment I, on aerobic stability was tested by treating silages prior to aerobic stability measurements. The novel lactic acid bacteria inoculant was equally effective in improving fermentation quality as the commercial inoculant. However, the aerobic stability of both inoculated silages was poorer than that of formic acid treated or the untreated one in one of the experiments. The results suggested that antimicrobial properties of E76 were not effective enough to improve aerobic instability. One option to overcome this problem is to use chemical additives in combination with the inoculants.
Original languageEnglish
Pages (from-to)185-199
JournalAgricultural and Food Science
Volume15
Issue number3
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

formic acid
Sorbic Acid
Sodium Benzoate
sodium benzoate
potassium sorbate
Silage
Benzoic Acid
grass silage
Poaceae
silage
Fermentation
lactic acid bacteria
Lactic Acid
fermentation
Bacteria
Festuca pratensis
Lactobacillus plantarum
anti-infective properties
preservatives

Keywords

  • silage making
  • grass silage
  • wilting
  • additives
  • aerobic stability,
  • inoculants
  • formic acid
  • fermentation
  • sodium benzoate
  • potassium
  • sorbate

Cite this

Saarisalo, Eeva ; Jalava, Taina ; Skyttä, Eija ; Haikara, Auli ; Jaakkola, Seija. / Effect of lactic acid bacteria inoculants, formic acid, potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage. In: Agricultural and Food Science. 2006 ; Vol. 15, No. 3. pp. 185-199.
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abstract = "The efficiency of a novel strain of lactic acid bacteria inoculant (Lactobacillus plantarum VTT E-78076, E76) on the fermentation quality of wilted silage was studied. Furthermore, the possibility to improve aerobic stability of silages by combining an inoculant and chemical preservatives was investigated. Two experiments were conducted with wilted timothy-meadow fescue herbage (dry matter 429 and 344 g kg-1) using six treatments. In experiment I, E76 (106 cfu g-1 fresh matter (FM)) was applied alone and in combination with sodium benzoate (0.3 g kg-1 grass FM) or low rate of formic acid (0.4 l t-1 FM). In experiment II, E76 and a commercial inoculant were applied alone and in combination with sodium benzoate. Untreated silage and formic acid (4 l t-1 FM) treated silage served as negative and positive controls in both experiments. The effect of sodium benzoate and potassium sorbate in experiment I, on aerobic stability was tested by treating silages prior to aerobic stability measurements. The novel lactic acid bacteria inoculant was equally effective in improving fermentation quality as the commercial inoculant. However, the aerobic stability of both inoculated silages was poorer than that of formic acid treated or the untreated one in one of the experiments. The results suggested that antimicrobial properties of E76 were not effective enough to improve aerobic instability. One option to overcome this problem is to use chemical additives in combination with the inoculants.",
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author = "Eeva Saarisalo and Taina Jalava and Eija Skytt{\"a} and Auli Haikara and Seija Jaakkola",
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language = "English",
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journal = "Agricultural and Food Science",
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Effect of lactic acid bacteria inoculants, formic acid, potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage. / Saarisalo, Eeva; Jalava, Taina; Skyttä, Eija; Haikara, Auli; Jaakkola, Seija.

In: Agricultural and Food Science, Vol. 15, No. 3, 2006, p. 185-199.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of lactic acid bacteria inoculants, formic acid, potassium sorbate and sodium benzoate on fermentation quality and aerobic stability of wilted grass silage

AU - Saarisalo, Eeva

AU - Jalava, Taina

AU - Skyttä, Eija

AU - Haikara, Auli

AU - Jaakkola, Seija

PY - 2006

Y1 - 2006

N2 - The efficiency of a novel strain of lactic acid bacteria inoculant (Lactobacillus plantarum VTT E-78076, E76) on the fermentation quality of wilted silage was studied. Furthermore, the possibility to improve aerobic stability of silages by combining an inoculant and chemical preservatives was investigated. Two experiments were conducted with wilted timothy-meadow fescue herbage (dry matter 429 and 344 g kg-1) using six treatments. In experiment I, E76 (106 cfu g-1 fresh matter (FM)) was applied alone and in combination with sodium benzoate (0.3 g kg-1 grass FM) or low rate of formic acid (0.4 l t-1 FM). In experiment II, E76 and a commercial inoculant were applied alone and in combination with sodium benzoate. Untreated silage and formic acid (4 l t-1 FM) treated silage served as negative and positive controls in both experiments. The effect of sodium benzoate and potassium sorbate in experiment I, on aerobic stability was tested by treating silages prior to aerobic stability measurements. The novel lactic acid bacteria inoculant was equally effective in improving fermentation quality as the commercial inoculant. However, the aerobic stability of both inoculated silages was poorer than that of formic acid treated or the untreated one in one of the experiments. The results suggested that antimicrobial properties of E76 were not effective enough to improve aerobic instability. One option to overcome this problem is to use chemical additives in combination with the inoculants.

AB - The efficiency of a novel strain of lactic acid bacteria inoculant (Lactobacillus plantarum VTT E-78076, E76) on the fermentation quality of wilted silage was studied. Furthermore, the possibility to improve aerobic stability of silages by combining an inoculant and chemical preservatives was investigated. Two experiments were conducted with wilted timothy-meadow fescue herbage (dry matter 429 and 344 g kg-1) using six treatments. In experiment I, E76 (106 cfu g-1 fresh matter (FM)) was applied alone and in combination with sodium benzoate (0.3 g kg-1 grass FM) or low rate of formic acid (0.4 l t-1 FM). In experiment II, E76 and a commercial inoculant were applied alone and in combination with sodium benzoate. Untreated silage and formic acid (4 l t-1 FM) treated silage served as negative and positive controls in both experiments. The effect of sodium benzoate and potassium sorbate in experiment I, on aerobic stability was tested by treating silages prior to aerobic stability measurements. The novel lactic acid bacteria inoculant was equally effective in improving fermentation quality as the commercial inoculant. However, the aerobic stability of both inoculated silages was poorer than that of formic acid treated or the untreated one in one of the experiments. The results suggested that antimicrobial properties of E76 were not effective enough to improve aerobic instability. One option to overcome this problem is to use chemical additives in combination with the inoculants.

KW - silage making

KW - grass silage

KW - wilting

KW - additives

KW - aerobic stability,

KW - inoculants

KW - formic acid

KW - fermentation

KW - sodium benzoate

KW - potassium

KW - sorbate

U2 - 10.2137/145960606779216263

DO - 10.2137/145960606779216263

M3 - Article

VL - 15

SP - 185

EP - 199

JO - Agricultural and Food Science

JF - Agricultural and Food Science

SN - 1459-6067

IS - 3

ER -