Effect of lignin chemistry on non-productive enzyme adsorption in the enzymatic hydrolysis of lignocellulose

Jenni Rahikainen, Raquel Martin-Sampedro, Harri Heikkinen, Stella Rovio, Kaisa Marjamaa, Tarja Tamminen, Orlando J. Rojas, Kristiina Kruus (Corresponding author)

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review


    Lignin is an inhibitory compound in the enzymatic hydrolysis of lignocellulosic feedstocks. Non-productive enzyme adsorption onto lignin and subsequent enzyme inactivation is a major inhibitory mechanism that increases the enzyme dosage and thus the costs, it also restricts enzyme recycling especially with softwood feedstocks. The underlying mechanisms causing non-productive enzyme adsorption have remained mostly unsolved. This paper describes interactions of the major Trichoderma reesei cellobiohydrolase Cel7A and its
    catalytic core domain with well-characterized isolated lignins from different botanical origin, namely from spruce and wheat straw. Both non-treated and steam explosion (SE) pretreated lignocellulosic materials were used for lignin isolation and subsequent preparation of ultrathin lignin films for enzyme adsorption studies. We applied quartz crystal microbalance (QCM) technique for real-time monitoring of enzyme adsorption onto the different lignin films. Interestingly, more enzyme was bound onto the lignin films prepared of steam pretreated materials, whereas botanical origin of lignin had only a minor effect on enzyme binding.
    Original languageEnglish
    Title of host publicationProceedings of the 13th European Workshop on Lignocellulosics and Pulp
    Subtitle of host publicationEWLP 2014
    EditorsJosé Carlos del Río Andrade, Ana Gutiérrez Suárez, Jorge Rencoret, Ángel T. Martínez
    Place of PublicationSevilla
    Publication statusPublished - 2014
    MoE publication typeA4 Article in a conference publication
    Event13th European Workshop on Lignocellulosics and Pulp, EWLP 2014 - Sevilla, Spain
    Duration: 24 Jun 201427 Jun 2014
    Conference number: 13


    Conference13th European Workshop on Lignocellulosics and Pulp, EWLP 2014
    Abbreviated titleEWLP 2014


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