Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce

S. Kuittinen, Y. Puentes Rodriguez, M. Yang, M. Keinänen, O. Pastinen, M. Siika-aho, O. Turunen, A. Pappinen

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

The present study investigated the ability of pressurized microwave pretreatment to convert softwood lignocellulose to fermentable monosaccharides. Norway spruce lignocellulose was subjected to microwave pretreatment (600 and 1200 W) under high pressure at different temperatures. Microwave pretreatment at mild acid concentrations (0.05–0.1 % H2SO4), temperatures of 170 and 200 °C, and a very short incubation time (5 min) released 84–100 % of hemicellulosic monosaccharides (mannose, galactose, and xylose). In addition, minimal amounts of degradation products (5-(hydroxymethyl)-2-furaldehyde, levulinic acid) were formed. The highest yield of fermentable sugars was 75 %, after both the pressurized microwave pretreatment with conditions 0.05 % H2SO4/600 W/200 °C/5 min and enzymatic hydrolysis with 20 FPU Celluclast 1.5 L, 400 nkat of Novozyme 188, and polyethyleneglycol (PEG) 4000 (0.3 g/g of pretreated material). Results showed that already 0.05 % H2SO4 used in microwave pretreatment could effectively liberate hemicellulose monosaccharides without serious monosaccharide degradation and form a basis for enzymatic hydrolysis.

Original languageEnglish
Pages (from-to)344-354
Number of pages11
JournalBioenergy Research
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Mar 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
enzymatic hydrolysis
hemicellulose
Picea abies
monosaccharides
pretreatment
Microwaves
lignocellulose
levulinic acid
Degradation
hydroxymethylfurfural
Xylose
degradation
Acids
Softwoods
softwood
polyethylene glycol
mannose
xylose
Sugars

Keywords

  • Hemicellulose
  • Lignocellulose
  • Microwaves
  • Norway spruce
  • Pretreatment
  • Softwood

Cite this

Kuittinen, S. ; Rodriguez, Y. Puentes ; Yang, M. ; Keinänen, M. ; Pastinen, O. ; Siika-aho, M. ; Turunen, O. ; Pappinen, A. / Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce. In: Bioenergy Research. 2016 ; Vol. 9, No. 1. pp. 344-354.
@article{a5766fcf94454a46838d7916c668fe05,
title = "Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce",
abstract = "The present study investigated the ability of pressurized microwave pretreatment to convert softwood lignocellulose to fermentable monosaccharides. Norway spruce lignocellulose was subjected to microwave pretreatment (600 and 1200 W) under high pressure at different temperatures. Microwave pretreatment at mild acid concentrations (0.05–0.1 {\%} H2SO4), temperatures of 170 and 200 °C, and a very short incubation time (5 min) released 84–100 {\%} of hemicellulosic monosaccharides (mannose, galactose, and xylose). In addition, minimal amounts of degradation products (5-(hydroxymethyl)-2-furaldehyde, levulinic acid) were formed. The highest yield of fermentable sugars was 75 {\%}, after both the pressurized microwave pretreatment with conditions 0.05 {\%} H2SO4/600 W/200 °C/5 min and enzymatic hydrolysis with 20 FPU Celluclast 1.5 L, 400 nkat of Novozyme 188, and polyethyleneglycol (PEG) 4000 (0.3 g/g of pretreated material). Results showed that already 0.05 {\%} H2SO4 used in microwave pretreatment could effectively liberate hemicellulose monosaccharides without serious monosaccharide degradation and form a basis for enzymatic hydrolysis.",
keywords = "Hemicellulose, Lignocellulose, Microwaves, Norway spruce, Pretreatment, Softwood",
author = "S. Kuittinen and Rodriguez, {Y. Puentes} and M. Yang and M. Kein{\"a}nen and O. Pastinen and M. Siika-aho and O. Turunen and A. Pappinen",
year = "2016",
month = "3",
day = "1",
doi = "10.1007/s12155-015-9696-9",
language = "English",
volume = "9",
pages = "344--354",
journal = "Bioenergy Research",
issn = "1939-1234",
publisher = "Springer",
number = "1",

}

Kuittinen, S, Rodriguez, YP, Yang, M, Keinänen, M, Pastinen, O, Siika-aho, M, Turunen, O & Pappinen, A 2016, 'Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce', Bioenergy Research, vol. 9, no. 1, pp. 344-354. https://doi.org/10.1007/s12155-015-9696-9

Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce. / Kuittinen, S.; Rodriguez, Y. Puentes; Yang, M.; Keinänen, M.; Pastinen, O.; Siika-aho, M.; Turunen, O.; Pappinen, A.

In: Bioenergy Research, Vol. 9, No. 1, 01.03.2016, p. 344-354.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of Microwave-Assisted Pretreatment Conditions on Hemicellulose Conversion and Enzymatic Hydrolysis of Norway Spruce

AU - Kuittinen, S.

AU - Rodriguez, Y. Puentes

AU - Yang, M.

AU - Keinänen, M.

AU - Pastinen, O.

AU - Siika-aho, M.

AU - Turunen, O.

AU - Pappinen, A.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The present study investigated the ability of pressurized microwave pretreatment to convert softwood lignocellulose to fermentable monosaccharides. Norway spruce lignocellulose was subjected to microwave pretreatment (600 and 1200 W) under high pressure at different temperatures. Microwave pretreatment at mild acid concentrations (0.05–0.1 % H2SO4), temperatures of 170 and 200 °C, and a very short incubation time (5 min) released 84–100 % of hemicellulosic monosaccharides (mannose, galactose, and xylose). In addition, minimal amounts of degradation products (5-(hydroxymethyl)-2-furaldehyde, levulinic acid) were formed. The highest yield of fermentable sugars was 75 %, after both the pressurized microwave pretreatment with conditions 0.05 % H2SO4/600 W/200 °C/5 min and enzymatic hydrolysis with 20 FPU Celluclast 1.5 L, 400 nkat of Novozyme 188, and polyethyleneglycol (PEG) 4000 (0.3 g/g of pretreated material). Results showed that already 0.05 % H2SO4 used in microwave pretreatment could effectively liberate hemicellulose monosaccharides without serious monosaccharide degradation and form a basis for enzymatic hydrolysis.

AB - The present study investigated the ability of pressurized microwave pretreatment to convert softwood lignocellulose to fermentable monosaccharides. Norway spruce lignocellulose was subjected to microwave pretreatment (600 and 1200 W) under high pressure at different temperatures. Microwave pretreatment at mild acid concentrations (0.05–0.1 % H2SO4), temperatures of 170 and 200 °C, and a very short incubation time (5 min) released 84–100 % of hemicellulosic monosaccharides (mannose, galactose, and xylose). In addition, minimal amounts of degradation products (5-(hydroxymethyl)-2-furaldehyde, levulinic acid) were formed. The highest yield of fermentable sugars was 75 %, after both the pressurized microwave pretreatment with conditions 0.05 % H2SO4/600 W/200 °C/5 min and enzymatic hydrolysis with 20 FPU Celluclast 1.5 L, 400 nkat of Novozyme 188, and polyethyleneglycol (PEG) 4000 (0.3 g/g of pretreated material). Results showed that already 0.05 % H2SO4 used in microwave pretreatment could effectively liberate hemicellulose monosaccharides without serious monosaccharide degradation and form a basis for enzymatic hydrolysis.

KW - Hemicellulose

KW - Lignocellulose

KW - Microwaves

KW - Norway spruce

KW - Pretreatment

KW - Softwood

UR - http://www.scopus.com/inward/record.url?scp=84959160065&partnerID=8YFLogxK

U2 - 10.1007/s12155-015-9696-9

DO - 10.1007/s12155-015-9696-9

M3 - Article

VL - 9

SP - 344

EP - 354

JO - Bioenergy Research

JF - Bioenergy Research

SN - 1939-1234

IS - 1

ER -