Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Kirsi Marja Oksman-Caldentey, Nina Sevón, Leena Vanhala, Raimo Hiltunen

Research output: Contribution to journalArticleScientificpeer-review

44 Citations (Scopus)

Abstract

The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g l-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg l-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-1 of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalPlant Cell, Tissue and Organ Culture
Volume38
Issue number2-3
DOIs
Publication statusPublished - 1 Sep 1994
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus muticus
sucrose
clones
metabolism
atropine
nitrogen
culture media
fatty acid composition
carbon
lipids
lactose
fructose
fatty acids
glucose
synthesis
biomass
nutrients

Keywords

  • Fatty acids
  • hyoscyamine
  • Hyoscyamus muticus
  • nitrogen
  • sucrose
  • transformed root cultures

Cite this

@article{a9d9d497b87e4fe0a98af017c00617ac,
title = "Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus",
abstract = "The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g l-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg l-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-1 of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.",
keywords = "Fatty acids, hyoscyamine, Hyoscyamus muticus, nitrogen, sucrose, transformed root cultures",
author = "Oksman-Caldentey, {Kirsi Marja} and Nina Sev{\'o}n and Leena Vanhala and Raimo Hiltunen",
year = "1994",
month = "9",
day = "1",
doi = "10.1007/BF00033886",
language = "English",
volume = "38",
pages = "263--272",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
publisher = "Springer",
number = "2-3",

}

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus. / Oksman-Caldentey, Kirsi Marja; Sevón, Nina; Vanhala, Leena; Hiltunen, Raimo.

In: Plant Cell, Tissue and Organ Culture, Vol. 38, No. 2-3, 01.09.1994, p. 263-272.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

AU - Oksman-Caldentey, Kirsi Marja

AU - Sevón, Nina

AU - Vanhala, Leena

AU - Hiltunen, Raimo

PY - 1994/9/1

Y1 - 1994/9/1

N2 - The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g l-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg l-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-1 of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.

AB - The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g l-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg l-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-1 of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.

KW - Fatty acids

KW - hyoscyamine

KW - Hyoscyamus muticus

KW - nitrogen

KW - sucrose

KW - transformed root cultures

UR - http://www.scopus.com/inward/record.url?scp=0000926398&partnerID=8YFLogxK

U2 - 10.1007/BF00033886

DO - 10.1007/BF00033886

M3 - Article

AN - SCOPUS:0000926398

VL - 38

SP - 263

EP - 272

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 2-3

ER -