TY - JOUR
T1 - Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract
T2 - A human cross over trial
AU - Hakkola, Salla
AU - Nylund, Lotta
AU - Rosa-Sibakov, Natalia
AU - Yang, Baoru
AU - Nordlund, Emilia
AU - Pahikkala, Tapio
AU - Kalliomäki, Marko
AU - Aura, Anna Marja
AU - Linderborg, Kaisa M.
N1 - Funding Information:
All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Seppänen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH).
Funding Information:
All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Sepp?nen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/4/16
Y1 - 2021/4/16
N2 - While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.
AB - While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.
KW - Avenanthramides
KW - Beta-glucan
KW - Bile acids
KW - Enzyme treatment
KW - Gastrointestinal pressure
KW - Molecular weight
KW - Oat
KW - Phenolic acids
UR - http://www.scopus.com/inward/record.url?scp=85092912498&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2020.128219
DO - 10.1016/j.foodchem.2020.128219
M3 - Article
AN - SCOPUS:85092912498
SN - 0308-8146
VL - 342
JO - Food Chemistry
JF - Food Chemistry
M1 - 128219
ER -