Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract: A human cross over trial

Salla Hakkola; Lotta Nylund; Natalia Rosa-Sibakov; Baoru Yang; Emilia Nordlund; Tapio Pahikkala; Marko Kalliomäki; Anna Marja Aura; Kaisa M. Linderborg

doi:10.1016/j.foodchem.2020.128219

Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract: A human cross over trial

Salla Hakkola
, Lotta Nylund
, Natalia Rosa-Sibakov
, Baoru Yang
, Emilia Nordlund
, Tapio Pahikkala
, Marko Kalliomäki
, Anna Marja Aura
, Kaisa M. Linderborg^*

^*Corresponding author for this work

BA53 Industrial biotechnology and food

University of Turku

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

Abstract

While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.

Original language	English
Article number	128219
Journal	Food Chemistry
Volume	342
DOIs	https://doi.org/10.1016/j.foodchem.2020.128219
Publication status	Published - 16 Apr 2021
MoE publication type	A1 Journal article-refereed

Funding

All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Seppänen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH).

Keywords

Avenanthramides
Beta-glucan
Bile acids
Enzyme treatment
Gastrointestinal pressure
Molecular weight
Oat
Phenolic acids

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10.1016/j.foodchem.2020.128219

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Hakkola, S., Nylund, L., Rosa-Sibakov, N., Yang, B., Nordlund, E., Pahikkala, T., Kalliomäki, M., Aura, A. M., & Linderborg, K. M. (2021). Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract: A human cross over trial. Food Chemistry, 342, Article 128219. https://doi.org/10.1016/j.foodchem.2020.128219

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title = "Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract: A human cross over trial",

abstract = "While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.",

keywords = "Avenanthramides, Beta-glucan, Bile acids, Enzyme treatment, Gastrointestinal pressure, Molecular weight, Oat, Phenolic acids",

author = "Salla Hakkola and Lotta Nylund and Natalia Rosa-Sibakov and Baoru Yang and Emilia Nordlund and Tapio Pahikkala and Marko Kalliom{\"a}ki and Aura, \{Anna Marja\} and Linderborg, \{Kaisa M.\}",

note = "Funding Information: All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Sepp{\"a}nen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH). Funding Information: All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Sepp?nen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH). Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = apr,

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doi = "10.1016/j.foodchem.2020.128219",

language = "English",

volume = "342",

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TY - JOUR

T1 - Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract

T2 - A human cross over trial

AU - Hakkola, Salla

AU - Nylund, Lotta

AU - Rosa-Sibakov, Natalia

AU - Yang, Baoru

AU - Nordlund, Emilia

AU - Pahikkala, Tapio

AU - Kalliomäki, Marko

AU - Aura, Anna Marja

AU - Linderborg, Kaisa M.

N1 - Funding Information: All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Seppänen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH). Funding Information: All the volunteers who participated in this study are warmly acknowledged. Outi Mattila is thankfully acknowledged for helping with the preparation of the oat bran concentrates. Heli Nygren, Tuulikki Sepp?nen-Laakso and Natalia Maiorova are acknowledged for their assistance with the analysis of the oat-derived phenolic compounds. Sanni Kivinen is thanked for her assistance in the bile acid analyses. Jukka-Pekka Suomela and Johanna Jokioja are thanked for assistance with the UHPLC-QTOF system. Elizabeth Nyman is thanked for proofreading the English language. This work was funded by Business Finland as part of the OATyourGUT project (grant number 5469/31/2016) co-funded by Finnish food companies and the University of Turku and by the Magnus Ehrnrooth Foundation (personal grant for SH). Publisher Copyright: © 2020 Elsevier Ltd Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4/16

Y1 - 2021/4/16

N2 - While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.

AB - While the development of oat products often requires altered molecular weight (MW) of β-glucan, the resulting health implications are currently unclear. This 3-leg crossover trial (n = 14) investigated the effects of the consumption of oat bran with High, Medium and Low MW β-glucan (average > 1000, 524 and 82 kDa respectively) with 3 consequent meals on oat-derived phenolic compounds in urine (UHPLC-MS/MS), bile acids in feces (UHPLC-QTOF), gastrointestinal conditions (ingestible capsule), and perceived gut well-being. Urine excretion of ferulic acid was higher (p < 0.001, p < 0.001), and the fecal excretion of deoxycholic (p < 0.03, p < 0.02) and chenodeoxycholic (p < 0.06, p < 0.02) acids lower after consumption of Low MW β-glucan compared with both Medium and High MW β-glucan. Duodenal pressure was higher after consumption of High MW β-glucan compared to Medium (p < 0.041) and Low (p < 0.022) MW β-glucan. The MW of β-glucan did not affect gut well-being, but the perceptions between females and males differed.

KW - Avenanthramides

KW - Beta-glucan

KW - Bile acids

KW - Enzyme treatment

KW - Gastrointestinal pressure

KW - Molecular weight

KW - Oat

KW - Phenolic acids

UR - https://www.scopus.com/pages/publications/85092912498

U2 - 10.1016/j.foodchem.2020.128219

DO - 10.1016/j.foodchem.2020.128219

M3 - Article

AN - SCOPUS:85092912498

SN - 0308-8146

VL - 342

JO - Food Chemistry

JF - Food Chemistry

M1 - 128219

ER -

Effect of oat β-glucan of different molecular weights on fecal bile acids, urine metabolites and pressure in the digestive tract: A human cross over trial

Abstract

Funding

Keywords

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