Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

Timo J. Hakala (Corresponding Author), Päivi Laaksonen, Aino Helle, Markus Linder, Kenneth Holmberg

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.
Original languageEnglish
Pages (from-to)241-247
Number of pages6
JournalTribology: Materials, Surfaces & Interfaces
Volume8
Issue number4
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Lubrication
Friction
Water
Stainless Steel
Ionic strength
Proteins
Buffers
Stainless steel
Wear of materials
Ellipsometry

Keywords

  • water based lubrication
  • hydrophobins
  • biolubrication
  • tribology

Cite this

Hakala, Timo J. ; Laaksonen, Päivi ; Helle, Aino ; Linder, Markus ; Holmberg, Kenneth. / Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems. In: Tribology: Materials, Surfaces & Interfaces. 2014 ; Vol. 8, No. 4. pp. 241-247.
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title = "Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems",
abstract = "In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38{\%} and wear by 40-65{\%} compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.",
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author = "Hakala, {Timo J.} and P{\"a}ivi Laaksonen and Aino Helle and Markus Linder and Kenneth Holmberg",
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Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems. / Hakala, Timo J. (Corresponding Author); Laaksonen, Päivi; Helle, Aino; Linder, Markus; Holmberg, Kenneth.

In: Tribology: Materials, Surfaces & Interfaces, Vol. 8, No. 4, 2014, p. 241-247.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

AU - Hakala, Timo J.

AU - Laaksonen, Päivi

AU - Helle, Aino

AU - Linder, Markus

AU - Holmberg, Kenneth

N1 - Project code: SA_BIOWAL 34126 Project code: NewLub 41202

PY - 2014

Y1 - 2014

N2 - In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.

AB - In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.

KW - water based lubrication

KW - hydrophobins

KW - biolubrication

KW - tribology

U2 - 10.1179/1751584X14Y.0000000084

DO - 10.1179/1751584X14Y.0000000084

M3 - Article

VL - 8

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EP - 247

JO - Tribology: Materials, Surfaces & Interfaces

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