Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

Timo J. Hakala (Corresponding Author), Päivi Laaksonen, Aino Helle, Markus Linder, Kenneth Holmberg

    Research output: Contribution to journalArticleScientificpeer-review

    5 Citations (Scopus)

    Abstract

    In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.
    Original languageEnglish
    Pages (from-to)241-247
    Number of pages6
    JournalTribology: Materials, Surfaces & Interfaces
    Volume8
    Issue number4
    DOIs
    Publication statusPublished - 2014
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Lubrication
    Friction
    Water
    Stainless Steel
    Ionic strength
    Proteins
    Buffers
    Stainless steel
    Wear of materials
    Ellipsometry

    Keywords

    • water based lubrication
    • hydrophobins
    • biolubrication
    • tribology

    Cite this

    Hakala, Timo J. ; Laaksonen, Päivi ; Helle, Aino ; Linder, Markus ; Holmberg, Kenneth. / Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems. In: Tribology: Materials, Surfaces & Interfaces. 2014 ; Vol. 8, No. 4. pp. 241-247.
    @article{448e21f688c043eda712dadde70fed0c,
    title = "Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems",
    abstract = "In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38{\%} and wear by 40-65{\%} compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.",
    keywords = "water based lubrication, hydrophobins, biolubrication, tribology",
    author = "Hakala, {Timo J.} and P{\"a}ivi Laaksonen and Aino Helle and Markus Linder and Kenneth Holmberg",
    note = "Project code: SA_BIOWAL 34126 Project code: NewLub 41202",
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    language = "English",
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    Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems. / Hakala, Timo J. (Corresponding Author); Laaksonen, Päivi; Helle, Aino; Linder, Markus; Holmberg, Kenneth.

    In: Tribology: Materials, Surfaces & Interfaces, Vol. 8, No. 4, 2014, p. 241-247.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Effect of operational conditions and environment on lubricity of hydrophobins in water based lubrication systems

    AU - Hakala, Timo J.

    AU - Laaksonen, Päivi

    AU - Helle, Aino

    AU - Linder, Markus

    AU - Holmberg, Kenneth

    N1 - Project code: SA_BIOWAL 34126 Project code: NewLub 41202

    PY - 2014

    Y1 - 2014

    N2 - In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.

    AB - In this study, the effect of operational conditions (normal load, sliding velocity) and environment (pH and ionic strength) on the lubrication properties of two different hydrophobin proteins were investigated using pin on disc tribometry and ellipsometry. The studied proteins were wild type HFBI and the glycosylated hydrophobin FpHYD5. It was observed that the friction of a stainless steel versus stainless steel contact lubricated with either of the hydrophobins did not depend on the normal load. However, increased sliding velocity occasionally led to a decrease in friction when the surfaces were lubricated with the glycosylated FpHYD5. The tribological behaviour of FpHYD5 was studied at pH values ranging from 3 to 9 and generally lowered friction by 31-38% and wear by 40-65% compared to the corresponding buffer solutions. An exception was pH 9, where FpHYD5 increased friction and wear compared to the buffer solution. Ionic strength affected both the amount of protein that was adsorbed and the lubrication properties of glycosylated hydrophobins.

    KW - water based lubrication

    KW - hydrophobins

    KW - biolubrication

    KW - tribology

    U2 - 10.1179/1751584X14Y.0000000084

    DO - 10.1179/1751584X14Y.0000000084

    M3 - Article

    VL - 8

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    EP - 247

    JO - Tribology: Materials, Surfaces & Interfaces

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    SN - 1751-5831

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