Effect of purified Trichoderma reesei cellulases on formation of cotton powder from cotton fabric

Lea Heikinheimo (Corresponding Author), Arja Miettinen-Oinonen, Artur Cavaco-Paulo, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG‐treated fabrics was clearly higher than the weight loss of CBH‐treated fabrics with both low and high mechanical action levels.
Original languageEnglish
Pages (from-to)1917-1922
Number of pages6
JournalJournal of Applied Polymer Science
Volume90
Issue number7
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulases
Cotton fabrics
Powders
Cotton
Oligosaccharides
Cellulase
Molecular weight
Sugars
Cellulose 1,4-beta-Cellobiosidase
Enzymes

Keywords

  • enzymes
  • molecular weight distribution
  • fibers
  • high performance liquid chromatography (HPLC)

Cite this

Heikinheimo, Lea ; Miettinen-Oinonen, Arja ; Cavaco-Paulo, Artur ; Buchert, Johanna. / Effect of purified Trichoderma reesei cellulases on formation of cotton powder from cotton fabric. In: Journal of Applied Polymer Science. 2003 ; Vol. 90, No. 7. pp. 1917-1922.
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abstract = "The mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG‐treated fabrics was clearly higher than the weight loss of CBH‐treated fabrics with both low and high mechanical action levels.",
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Heikinheimo, L, Miettinen-Oinonen, A, Cavaco-Paulo, A & Buchert, J 2003, 'Effect of purified Trichoderma reesei cellulases on formation of cotton powder from cotton fabric', Journal of Applied Polymer Science, vol. 90, no. 7, pp. 1917-1922. https://doi.org/10.1002/app.12868

Effect of purified Trichoderma reesei cellulases on formation of cotton powder from cotton fabric. / Heikinheimo, Lea (Corresponding Author); Miettinen-Oinonen, Arja; Cavaco-Paulo, Artur; Buchert, Johanna.

In: Journal of Applied Polymer Science, Vol. 90, No. 7, 2003, p. 1917-1922.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The mode of action of monocomponent purified Trichoderma reesei cellobiohydrolases (CBHI and CBHII) and endoglucanases (EGI and EGII) on cotton fabrics was studied by analyzing the weight loss of the fabric, the reducing sugars, the soluble oligosaccharides and the molecular weight of the cotton powder formed. The impact of mechanical action on these factors was also evaluated. EGI and EGII released the highest amounts of reducing sugars and soluble oligosaccharides in both treatments with or without additional mechanical action. After cellulase treatment without additional mechanical action, all of the cellulases were found to have reduced the molecular weight of cotton poplin powder. When mechanical action was combined with enzyme treatments, only EGII reduced the molecular weight. The weight loss of EG‐treated fabrics was clearly higher than the weight loss of CBH‐treated fabrics with both low and high mechanical action levels.

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