Effect of side groups on the action of β-xylosidase from Trichoderma reesei against substituted xylo-oligosaccharides

Maija Tenkanen (Corresponding Author), Elina Luonteri, Anita Teleman

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

Abstract

The action of β-xylosidase from Trichoderma reesei against different substituted xylo-oligosaccharides was studied. The enzyme cleaved off all unsubstituted xylose units from the non-reducing end of 1,2-linked uronic acid substituted xylo-oligosaccharides. Surprisingly, an l-arabinofuranosyl group linked α-1,3 to the xylopyranosyl ring was found to protect the β-1,4-xylosidic linkage before the substituted xylose unit from being cleaved by the β-xylosidase. Most probably the 1,3-linked substituent sterically hinders the hydrolysis. According to the results of the present work, β-xylosidase of T. reesei is not able to remove all unsubstituted xylose units from the non-reducing end of substituted xylo-oligosaccharides, as had been believed previously.
Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalFEBS Letters
Volume399
Issue number3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Xylosidases
Trichoderma
Xylose
Oligosaccharides
Uronic Acids
Hydrolysis
Enzymes

Cite this

Tenkanen, Maija ; Luonteri, Elina ; Teleman, Anita. / Effect of side groups on the action of β-xylosidase from Trichoderma reesei against substituted xylo-oligosaccharides. In: FEBS Letters. 1996 ; Vol. 399, No. 3. pp. 303-306.
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Effect of side groups on the action of β-xylosidase from Trichoderma reesei against substituted xylo-oligosaccharides. / Tenkanen, Maija (Corresponding Author); Luonteri, Elina; Teleman, Anita.

In: FEBS Letters, Vol. 399, No. 3, 1996, p. 303-306.

Research output: Contribution to journalArticleScientificpeer-review

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