Effects of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors

Walter van Gulik, Anna-Maria Nuutila, Ko L. Vinke, Hens Hoopen ten, Joseph Heijnen

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    A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth-min−1 (vvm) nor CO2 enrichment of the aeration gas had any effect on the growth of the cells.
    Original languageEnglish
    Pages (from-to)335-339
    Number of pages5
    JournalBiotechnology Progress
    Issue number3
    Publication statusPublished - 1994
    MoE publication typeA1 Journal article-refereed


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