Effects of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors

Walter van Gulik, Anna-Maria Nuutila, Ko L. Vinke, Hens Hoopen ten, Joseph Heijnen

    Research output: Contribution to journalArticleScientificpeer-review

    15 Citations (Scopus)

    Abstract

    A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth-min−1 (vvm) nor CO2 enrichment of the aeration gas had any effect on the growth of the cells.
    Original languageEnglish
    Pages (from-to)335-339
    Number of pages5
    JournalBiotechnology Progress
    Volume10
    Issue number3
    DOIs
    Publication statusPublished - 1994
    MoE publication typeA1 Journal article-refereed

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    Catharanthus
    Bioreactors
    Carbon Dioxide
    Suspensions
    Air
    Growth
    Conditioned Culture Medium
    Gases

    Cite this

    @article{a23e5c604c8649939e82ee912b282ca1,
    title = "Effects of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors",
    abstract = "A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth-min−1 (vvm) nor CO2 enrichment of the aeration gas had any effect on the growth of the cells.",
    author = "Gulik, {Walter van} and Anna-Maria Nuutila and Vinke, {Ko L.} and {Hoopen ten}, Hens and Joseph Heijnen",
    note = "Project code: BIO9001",
    year = "1994",
    doi = "10.1021/bp00027a015",
    language = "English",
    volume = "10",
    pages = "335--339",
    journal = "Biotechnology Progress",
    issn = "8756-7938",
    publisher = "Wiley",
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    Effects of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors. / Gulik, Walter van; Nuutila, Anna-Maria; Vinke, Ko L.; Hoopen ten, Hens; Heijnen, Joseph.

    In: Biotechnology Progress, Vol. 10, No. 3, 1994, p. 335-339.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Effects of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors

    AU - Gulik, Walter van

    AU - Nuutila, Anna-Maria

    AU - Vinke, Ko L.

    AU - Hoopen ten, Hens

    AU - Heijnen, Joseph

    N1 - Project code: BIO9001

    PY - 1994

    Y1 - 1994

    N2 - A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth-min−1 (vvm) nor CO2 enrichment of the aeration gas had any effect on the growth of the cells.

    AB - A high reproducibility of batch growth of Catharanthus roseus could only be achieved by using identical inoculum material. It was found that the initial growth rate of the cells increased with increasing inoculation density, whereas the maximum growth rate was not affected. Withdrawal of the conditioned medium from the inoculum resulted in a strong decline in the initial growth rate, indicating that conditioning factors affected the initial growth rate. Neither increasing the air flow rate from 0.5 to 1.5 volume of air per volume of broth-min−1 (vvm) nor CO2 enrichment of the aeration gas had any effect on the growth of the cells.

    U2 - 10.1021/bp00027a015

    DO - 10.1021/bp00027a015

    M3 - Article

    VL - 10

    SP - 335

    EP - 339

    JO - Biotechnology Progress

    JF - Biotechnology Progress

    SN - 8756-7938

    IS - 3

    ER -