Abstract
| Original language | English |
|---|---|
| Number of pages | 11 |
| Journal | Nutrition and Metabolism |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 |
| MoE publication type | Not Eligible |
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Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur. / Ma, Hongqiang; Turpeinen, Tuomas; Silvennoinen, Mika; Torvinen, Sira; Rinnankoski-Tuikka, Rita; Kainulainen, Heikki; Timonen, Jussi; Kujala, Urho M.; Rahkila, Paavo; Suominen, Harri.
In: Nutrition and Metabolism, Vol. 8, No. 1, 2011.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur
AU - Ma, Hongqiang
AU - Turpeinen, Tuomas
AU - Silvennoinen, Mika
AU - Torvinen, Sira
AU - Rinnankoski-Tuikka, Rita
AU - Kainulainen, Heikki
AU - Timonen, Jussi
AU - Kujala, Urho M.
AU - Rahkila, Paavo
AU - Suominen, Harri
PY - 2011
Y1 - 2011
N2 - Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth
AB - Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth
U2 - 10.1186/1743-7075-8-1
DO - 10.1186/1743-7075-8-1
M3 - Article
C2 - 21241467
VL - 8
JO - Annals of Nutrition and Metabolism
JF - Annals of Nutrition and Metabolism
SN - 0250-6807
IS - 1
ER -