Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur

Hongqiang Ma, Tuomas Turpeinen, Mika Silvennoinen, Sira Torvinen, Rita Rinnankoski-Tuikka, Heikki Kainulainen, Jussi Timonen, Urho M. Kujala, Paavo Rahkila, Harri Suominen

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)


Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth
Original languageEnglish
Number of pages11
JournalNutrition and Metabolism
Issue number1
Publication statusPublished - 2011
MoE publication typeNot Eligible


Dive into the research topics of 'Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur'. Together they form a unique fingerprint.

Cite this