Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur

Hongqiang Ma, Tuomas Turpeinen, Mika Silvennoinen, Sira Torvinen, Rita Rinnankoski-Tuikka, Heikki Kainulainen, Jussi Timonen, Urho M. Kujala, Paavo Rahkila, Harri Suominen

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth
Original languageEnglish
Number of pages11
JournalNutrition and Metabolism
Volume8
Issue number1
DOIs
Publication statusPublished - 2011
MoE publication typeNot Eligible

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High Fat Diet
Running
Femur
Obesity
Diet
Tomography
Bone and Bones
Obese Mice
Bone Development
Thigh
Inbred C57BL Mouse
Osteoporosis
Weight Gain
Minerals
Insulin Resistance
Adipose Tissue
Life Style
Control Groups
Health

Cite this

Ma, Hongqiang ; Turpeinen, Tuomas ; Silvennoinen, Mika ; Torvinen, Sira ; Rinnankoski-Tuikka, Rita ; Kainulainen, Heikki ; Timonen, Jussi ; Kujala, Urho M. ; Rahkila, Paavo ; Suominen, Harri. / Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur. In: Nutrition and Metabolism. 2011 ; Vol. 8, No. 1.
@article{2509ace0c924464ca009b6c80c29e571,
title = "Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur",
abstract = "Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70{\%} for HF and 60{\%} for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth",
author = "Hongqiang Ma and Tuomas Turpeinen and Mika Silvennoinen and Sira Torvinen and Rita Rinnankoski-Tuikka and Heikki Kainulainen and Jussi Timonen and Kujala, {Urho M.} and Paavo Rahkila and Harri Suominen",
year = "2011",
doi = "10.1186/1743-7075-8-1",
language = "English",
volume = "8",
journal = "Annals of Nutrition and Metabolism",
issn = "0250-6807",
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Ma, H, Turpeinen, T, Silvennoinen, M, Torvinen, S, Rinnankoski-Tuikka, R, Kainulainen, H, Timonen, J, Kujala, UM, Rahkila, P & Suominen, H 2011, 'Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur', Nutrition and Metabolism, vol. 8, no. 1. https://doi.org/10.1186/1743-7075-8-1

Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur. / Ma, Hongqiang; Turpeinen, Tuomas; Silvennoinen, Mika; Torvinen, Sira; Rinnankoski-Tuikka, Rita; Kainulainen, Heikki; Timonen, Jussi; Kujala, Urho M.; Rahkila, Paavo; Suominen, Harri.

In: Nutrition and Metabolism, Vol. 8, No. 1, 2011.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effects of diet-induced obesity and voluntary wheel running on the microstructure of the murine distal femur

AU - Ma, Hongqiang

AU - Turpeinen, Tuomas

AU - Silvennoinen, Mika

AU - Torvinen, Sira

AU - Rinnankoski-Tuikka, Rita

AU - Kainulainen, Heikki

AU - Timonen, Jussi

AU - Kujala, Urho M.

AU - Rahkila, Paavo

AU - Suominen, Harri

PY - 2011

Y1 - 2011

N2 - Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth

AB - Background: Obesity and osteoporosis, two possibly related conditions, are rapidly expanding health concerns in modern society. Both of them are associated with sedentary life style and nutrition. To investigate the effects of diet-induced obesity and voluntary physical activity we used high resolution micro-computed tomography (μCT) together with peripheral quantitative computed tomography (pQCT) to examine the microstructure of the distal femoral metaphysis in mice. Methods: Forty 7-week-old male C57BL/6J mice were assigned to 4 groups: control (C), control + running (CR), high-fat diet (HF), and high-fat diet + running (HFR). After a 21-week intervention, all the mice were sacrificed and the left femur dissected for pQCT and μCT measurements. Results: The mice fed the high-fat diet showed a significant weight gain (over 70% for HF and 60% for HFR), with increased epididymal fat pad mass and impaired insulin sensitivity. These obese mice had significantly higher trabecular connectivity density, volume, number, thickness, area and mass, and smaller trabecular separation. At the whole bone level, they had larger bone circumference and cross-sectional area and higher density-weighted maximal, minimal, and polar moments of inertia. Voluntary wheel running decreased all the cortical bone parameters, but increased the trabecular mineral density, and decreased the pattern factor and structure model index towards a more plate-like structure. Conclusions: The results suggest that in mice the femur adapts to obesity by improving bone strength both at the whole bone and micro-structural level. Adaptation to running exercise manifests itself in increased trabecular density and improved 3D structure, but in a limited overall bone growth

U2 - 10.1186/1743-7075-8-1

DO - 10.1186/1743-7075-8-1

M3 - Article

VL - 8

JO - Annals of Nutrition and Metabolism

JF - Annals of Nutrition and Metabolism

SN - 0250-6807

IS - 1

ER -