Effects of disintegration on in vitro fermentation and conversion patterns of wheat aleurone in a metabolical colon model

N.N. Rosa, AM Aura, Luc Saulnier, Ulla Holopainen-Mantila, Kaisa Poutanen, Valérie Micard (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

This work aimed to elucidate the effect of wheat aleurone integrity on its fermentability, i.e., the formation of short-chain fatty acids (SCFA) and microbial phenolic metabolites, in an in vitro model using human faecal microbiota as an inoculum. The structure of aleurone was modified by mechanical (dry grinding) or enzymatic (xylanase with or without feruloyl esterase) treatments in order to increase its physical accessibility and degrade its complex cell-wall network. The ground aleurone (smaller particle size) produced slightly more SCFA than the native aleurone during the first 8 h but a similar amount at 24 h (102.5 and 101 mmol/L, respectively). Similar colonic metabolism of ferulic acid (FA) was observed for native and ground aleurone. The enzymatic treatments of aleurone allowed a high solubilization of arabinoxylan (up to 82%) and a high release of FA in its conjugated and free forms (up to 87%). The enzymatic disintegration of aleurone's structure led to a higher concentration and formation rate of the colonic metabolites of FA (especially phenylpropionic acids) but did not change significantly the formation of SCFA (81 mmol/L for enzyme treated versus 101 mmol/L for the native aleurone).
Original languageEnglish
Pages (from-to)5805-5816
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume61
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

ferulic acid
Volatile Fatty Acids
Disintegration
short chain fatty acids
Fermentation
Triticum
colon
Colon
fermentation
Metabolites
wheat
metabolites
arabinoxylan
Microbiota
enzymatic treatment
grinding
xylanases
solubilization
Particle Size
Metabolism

Keywords

  • aleurone
  • ferulic acid
  • feruloyl esterase
  • particl
  • particle size
  • short-chain fatty acids
  • xylanase treatment

Cite this

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title = "Effects of disintegration on in vitro fermentation and conversion patterns of wheat aleurone in a metabolical colon model",
abstract = "This work aimed to elucidate the effect of wheat aleurone integrity on its fermentability, i.e., the formation of short-chain fatty acids (SCFA) and microbial phenolic metabolites, in an in vitro model using human faecal microbiota as an inoculum. The structure of aleurone was modified by mechanical (dry grinding) or enzymatic (xylanase with or without feruloyl esterase) treatments in order to increase its physical accessibility and degrade its complex cell-wall network. The ground aleurone (smaller particle size) produced slightly more SCFA than the native aleurone during the first 8 h but a similar amount at 24 h (102.5 and 101 mmol/L, respectively). Similar colonic metabolism of ferulic acid (FA) was observed for native and ground aleurone. The enzymatic treatments of aleurone allowed a high solubilization of arabinoxylan (up to 82{\%}) and a high release of FA in its conjugated and free forms (up to 87{\%}). The enzymatic disintegration of aleurone's structure led to a higher concentration and formation rate of the colonic metabolites of FA (especially phenylpropionic acids) but did not change significantly the formation of SCFA (81 mmol/L for enzyme treated versus 101 mmol/L for the native aleurone).",
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author = "N.N. Rosa and AM Aura and Luc Saulnier and Ulla Holopainen-Mantila and Kaisa Poutanen and Val{\'e}rie Micard",
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Effects of disintegration on in vitro fermentation and conversion patterns of wheat aleurone in a metabolical colon model. / Rosa, N.N.; Aura, AM; Saulnier, Luc; Holopainen-Mantila, Ulla; Poutanen, Kaisa; Micard, Valérie (Corresponding Author).

In: Journal of Agricultural and Food Chemistry, Vol. 61, 2013, p. 5805-5816.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effects of disintegration on in vitro fermentation and conversion patterns of wheat aleurone in a metabolical colon model

AU - Rosa, N.N.

AU - Aura, AM

AU - Saulnier, Luc

AU - Holopainen-Mantila, Ulla

AU - Poutanen, Kaisa

AU - Micard, Valérie

PY - 2013

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N2 - This work aimed to elucidate the effect of wheat aleurone integrity on its fermentability, i.e., the formation of short-chain fatty acids (SCFA) and microbial phenolic metabolites, in an in vitro model using human faecal microbiota as an inoculum. The structure of aleurone was modified by mechanical (dry grinding) or enzymatic (xylanase with or without feruloyl esterase) treatments in order to increase its physical accessibility and degrade its complex cell-wall network. The ground aleurone (smaller particle size) produced slightly more SCFA than the native aleurone during the first 8 h but a similar amount at 24 h (102.5 and 101 mmol/L, respectively). Similar colonic metabolism of ferulic acid (FA) was observed for native and ground aleurone. The enzymatic treatments of aleurone allowed a high solubilization of arabinoxylan (up to 82%) and a high release of FA in its conjugated and free forms (up to 87%). The enzymatic disintegration of aleurone's structure led to a higher concentration and formation rate of the colonic metabolites of FA (especially phenylpropionic acids) but did not change significantly the formation of SCFA (81 mmol/L for enzyme treated versus 101 mmol/L for the native aleurone).

AB - This work aimed to elucidate the effect of wheat aleurone integrity on its fermentability, i.e., the formation of short-chain fatty acids (SCFA) and microbial phenolic metabolites, in an in vitro model using human faecal microbiota as an inoculum. The structure of aleurone was modified by mechanical (dry grinding) or enzymatic (xylanase with or without feruloyl esterase) treatments in order to increase its physical accessibility and degrade its complex cell-wall network. The ground aleurone (smaller particle size) produced slightly more SCFA than the native aleurone during the first 8 h but a similar amount at 24 h (102.5 and 101 mmol/L, respectively). Similar colonic metabolism of ferulic acid (FA) was observed for native and ground aleurone. The enzymatic treatments of aleurone allowed a high solubilization of arabinoxylan (up to 82%) and a high release of FA in its conjugated and free forms (up to 87%). The enzymatic disintegration of aleurone's structure led to a higher concentration and formation rate of the colonic metabolites of FA (especially phenylpropionic acids) but did not change significantly the formation of SCFA (81 mmol/L for enzyme treated versus 101 mmol/L for the native aleurone).

KW - aleurone

KW - ferulic acid

KW - feruloyl esterase

KW - particl

KW - particle size

KW - short-chain fatty acids

KW - xylanase treatment

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DO - 10.1021/jf4001814

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JO - Journal of Agricultural and Food Chemistry

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