Effects of laccase-mediator combinations on wool

Raija Lantto, Christa Schönberg, Johanna Buchert, Elisabeth Heine

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Abstract

The effects of Myceliophthora thermophila laccase are studied alone and in combina tion with the mediators violuric acid (VA) or l-hydroxybenzotriazole (HBT) on wool fibers, cystine, and tyrosine. Without a mediator, laccase is unable to oxidize wool or the amino acids. In the presence of either VA or HBT, oxygen consumption is observed, indicating oxidation of the secondary substrates of the laccase, i.e., wool, cystine, or tyrosine. The oxidation levels are low. Laccase/HBT reacts more efficiently with wool and tyrosine and laccase/VA with cystine. With the chosen enzyme dosage and mediator concentration, about 2-4% of cystine and about 1 % of tyrosine are oxidized. The slight oxidation of wool fibers is not sufficient to affect the surface chemistry or alkaline solubility of the fibers, although staining of the fibers by the oxidized mediators is significant.
Original languageEnglish
Pages (from-to)713 - 717
Number of pages5
JournalTextile Reseach Journal
Volume74
Issue number8
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Cystines
Laccase
Wool
Cystine
Tyrosine
Wool fibers
Oxidation
Acids
Fibers
Surface chemistry
Amino acids
Enzymes
Solubility
Oxygen
Substrates
Amino Acids
violuric acid

Cite this

Lantto, Raija ; Schönberg, Christa ; Buchert, Johanna ; Heine, Elisabeth. / Effects of laccase-mediator combinations on wool. In: Textile Reseach Journal. 2004 ; Vol. 74, No. 8. pp. 713 - 717.
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Lantto, R, Schönberg, C, Buchert, J & Heine, E 2004, 'Effects of laccase-mediator combinations on wool', Textile Reseach Journal, vol. 74, no. 8, pp. 713 - 717. https://doi.org/10.1177/004051750407400809

Effects of laccase-mediator combinations on wool. / Lantto, Raija; Schönberg, Christa; Buchert, Johanna; Heine, Elisabeth.

In: Textile Reseach Journal, Vol. 74, No. 8, 2004, p. 713 - 717.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effects of laccase-mediator combinations on wool

AU - Lantto, Raija

AU - Schönberg, Christa

AU - Buchert, Johanna

AU - Heine, Elisabeth

PY - 2004

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AB - The effects of Myceliophthora thermophila laccase are studied alone and in combina tion with the mediators violuric acid (VA) or l-hydroxybenzotriazole (HBT) on wool fibers, cystine, and tyrosine. Without a mediator, laccase is unable to oxidize wool or the amino acids. In the presence of either VA or HBT, oxygen consumption is observed, indicating oxidation of the secondary substrates of the laccase, i.e., wool, cystine, or tyrosine. The oxidation levels are low. Laccase/HBT reacts more efficiently with wool and tyrosine and laccase/VA with cystine. With the chosen enzyme dosage and mediator concentration, about 2-4% of cystine and about 1 % of tyrosine are oxidized. The slight oxidation of wool fibers is not sufficient to affect the surface chemistry or alkaline solubility of the fibers, although staining of the fibers by the oxidized mediators is significant.

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DO - 10.1177/004051750407400809

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SP - 713

EP - 717

JO - Textile Reseach Journal

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SN - 0040-5175

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ER -