Effects of long-term preservation on growth and productivity of Panax ginseng and Catharanthus roseus cell cultures

Leena Mannonen, Leena Toivonen, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Cell cultures of Panax ginseng and Catharanthus roseus producing secondary metabolites were preserved in liquid nitrogen or under mineral oil for six months. The growth behaviour and the ability of the cultures to produce ginsenosides or indole alkaloids were measured after a recovery period and compared with cultures maintained by frequent subcultivation during the same period. Neither growth kinetics nor the degree of vacuolization during growth were affected by the long term preservation. Some changes in secondary metabolism were however found, indicating that preservation under mineral oil does not preserve the productivity of cell cultures whereas the cryogenic method does.
Original languageEnglish
Pages (from-to)173-177
JournalPlant Cell Reports
Volume9
Issue number4
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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Panax ginseng
mineral oil
Catharanthus roseus
long term effects
cell culture
indole alkaloids
secondary metabolites
preserves
kinetics
metabolism
liquids
nitrogen
methodology
ginsenosides
cryogenics

Cite this

Mannonen, Leena ; Toivonen, Leena ; Kauppinen, Veli. / Effects of long-term preservation on growth and productivity of Panax ginseng and Catharanthus roseus cell cultures. In: Plant Cell Reports. 1990 ; Vol. 9, No. 4. pp. 173-177.
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Effects of long-term preservation on growth and productivity of Panax ginseng and Catharanthus roseus cell cultures. / Mannonen, Leena; Toivonen, Leena; Kauppinen, Veli.

In: Plant Cell Reports, Vol. 9, No. 4, 1990, p. 173-177.

Research output: Contribution to journalArticleScientificpeer-review

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