Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation

Luis O. Morales; Mikhail Iakovlev; Raquel Martin-Sampedro; Jenni Rahikainen; Janne Laine; Adriaan van Heiningen; Orlando J. Rojas

doi:10.1016/j.biortech.2014.03.025

Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO₂-ethanol-water fractionation

Luis O. Morales, Mikhail Iakovlev, Raquel Martin-Sampedro, Jenni Rahikainen, Janne Laine, Adriaan van Heiningen, Orlando J. Rojas

Research output: Contribution to journal › Article › Scientific › peer-review

33 Citations (Scopus)

Abstract

The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO₂-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.

Original language	English
Pages (from-to)	55-62
Journal	Bioresource Technology
Volume	161
DOIs	https://doi.org/10.1016/j.biortech.2014.03.025
Publication status	Published - 2014
MoE publication type	A1 Journal article-refereed

Keywords

lignocellulose nanofibrils (LCNF)
SO2-ethanol-water (SEW)
bioconversion
residual lignin
hemicelluloses

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10.1016/j.biortech.2014.03.025

Cite this

@article{8d9e1b51d15a40d98a1bfb25b6df0d02,

title = "Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation",

abstract = "The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.",

keywords = "lignocellulose nanofibrils (LCNF), SO2-ethanol-water (SEW), bioconversion, residual lignin, hemicelluloses",

author = "Morales, {Luis O.} and Mikhail Iakovlev and Raquel Martin-Sampedro and Jenni Rahikainen and Janne Laine and {van Heiningen}, Adriaan and Rojas, {Orlando J.}",

year = "2014",

doi = "10.1016/j.biortech.2014.03.025",

language = "English",

volume = "161",

pages = "55--62",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

}

TY - JOUR

T1 - Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation

AU - Morales, Luis O.

AU - Iakovlev, Mikhail

AU - Martin-Sampedro, Raquel

AU - Rahikainen, Jenni

AU - Laine, Janne

AU - van Heiningen, Adriaan

AU - Rojas, Orlando J.

PY - 2014

Y1 - 2014

N2 - The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.

AB - The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.

KW - lignocellulose nanofibrils (LCNF)

KW - SO2-ethanol-water (SEW)

KW - bioconversion

KW - residual lignin

KW - hemicelluloses

U2 - 10.1016/j.biortech.2014.03.025

DO - 10.1016/j.biortech.2014.03.025

M3 - Article

SN - 0960-8524

VL - 161

SP - 55

EP - 62

JO - Bioresource Technology

JF - Bioresource Technology