Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation

L.O. Morales, M Iakovlev, R. Martin-Sampedro, Jenni Rahikainen, J. Laine, A. van Heiningen, O.J. Rojas

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.
Original languageEnglish
Pages (from-to)55-62
JournalBioresource Technology
Volume161
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Bioconversion
Softwoods
Lignin
Fractionation
lignin
ethanol
Ethanol
fractionation
Water
enzyme
Fibers
hydrolysis
Hydrolysis
Enzymes
Enzyme inhibition
Saccharification
water
Quartz crystal microbalances
Enzyme activity
digestibility

Keywords

  • lignocellulose nanofibrils (LCNF)
  • SO2-ethanol-water (SEW)
  • bioconversion
  • residual lignin
  • hemicelluloses

Cite this

Morales, L.O. ; Iakovlev, M ; Martin-Sampedro, R. ; Rahikainen, Jenni ; Laine, J. ; van Heiningen, A. ; Rojas, O.J. / Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation. In: Bioresource Technology. 2014 ; Vol. 161. pp. 55-62.
@article{8d9e1b51d15a40d98a1bfb25b6df0d02,
title = "Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation",
abstract = "The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.",
keywords = "lignocellulose nanofibrils (LCNF), SO2-ethanol-water (SEW), bioconversion, residual lignin, hemicelluloses",
author = "L.O. Morales and M Iakovlev and R. Martin-Sampedro and Jenni Rahikainen and J. Laine and {van Heiningen}, A. and O.J. Rojas",
year = "2014",
doi = "10.1016/j.biortech.2014.03.025",
language = "English",
volume = "161",
pages = "55--62",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier",

}

Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation. / Morales, L.O.; Iakovlev, M; Martin-Sampedro, R.; Rahikainen, Jenni; Laine, J.; van Heiningen, A.; Rojas, O.J.

In: Bioresource Technology, Vol. 161, 2014, p. 55-62.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effects of residual lignin and heteropolysaccharides on the bioconversion of softwood lignocellulose nanofibrils obtained by SO2-ethanol-water fractionation

AU - Morales, L.O.

AU - Iakovlev, M

AU - Martin-Sampedro, R.

AU - Rahikainen, Jenni

AU - Laine, J.

AU - van Heiningen, A.

AU - Rojas, O.J.

PY - 2014

Y1 - 2014

N2 - The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.

AB - The amount of residual lignin and hemicelluloses in softwood fibers was systematically varied by SO2-ethanol-water fractionation for integrated biorefinery with nanomaterial and biofuel production. On the basis of their low energy demand in mechanical processing, the fibers were deconstructed to lignocellulose nanofibrils (LCNF) and used as substrate for bioconversion. The effect of LCNF composition on saccharification via multicomponent enzymes was investigated at different loadings. LCNF digestibility was compared with the enzyme activity measured with a quartz crystal microbalance. LCNF hydrolysis rate gradually decreased with lignin and hemicellulose concentration, both of which limited enzyme accessibility. Enzyme inhibition resulted from non-productive binding of proteins onto lignin. Near complete LCNF hydrolysis was achieved, even at high lignin and hemicellulose content. Sugar yields for LCNF were higher than those for precursor SEW fibers, highlighting the benefits of high surface area in LCNF.

KW - lignocellulose nanofibrils (LCNF)

KW - SO2-ethanol-water (SEW)

KW - bioconversion

KW - residual lignin

KW - hemicelluloses

U2 - 10.1016/j.biortech.2014.03.025

DO - 10.1016/j.biortech.2014.03.025

M3 - Article

VL - 161

SP - 55

EP - 62

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -