Effects of various plant extracts on acid production by plaque and mutans streptococci

E. Söderling, S. Karjalainen, Martina Lille, Liisa Nohynek, Karin Autio, Maria Saarela

Research output: Contribution to journalOther journal contributionScientific

Abstract

The aim of this study was to compare the ability of selected plant extracts to inhibit acid production from starch gels by plaque in vivo and by mutans streptococci in vitro. Commercially available propolis, liquorice, green tea, grape fruit seed, black currant seed and cloudberry seed extracts were used in the study. The extracts were added to a starch gel, which consisted of 8% acid hydrolysed corn starch, 25% maltitol syrup and water (w/w). The pH of the gel was adjusted close to 6,5. For the in vivo acid production tests the subjects accumulated plaque for 2-3 days. After a single intake of 6 g of the test product, it was swished around in the mouth for 2 min. Changes of plaque pH in 2-3 interdental spaces were measured with a microelectrode (Beetrode) for 30 min. In the in vitro tests Streptococcus mutans NCTC 10449 was mixed with the gel suspensions and acid production was followed electrometrically for up to 10 min. Propolis inhibited acid production both in vitro and in vivo at ≥ 5%. Liquorice inhibited acid production at  1% in vitro and at  5.0 % in vivo. Grape fruit seed and green tea extracts prevented acid production completely: grape fruit seed in vitro at  0.5% and green tea in vitro at  0.1% and in vivo at 1.0 %. Berry seed oil extracts (1 %) had no effect on acid production. In conclusion, acid production was inhibited by several plant extracts, which makes them promising components of toothfriendly products. Financial support by the National Technology Agency (Tekes), Finland, is gratefully acknowledged.
Original languageEnglish
Pages (from-to)371
JournalCaries Research
Volume38
Publication statusPublished - 2004
MoE publication typeB1 Article in a scientific magazine
Event51st ORCA Congress - Marburg, Germany
Duration: 30 Jun 20043 Jul 2004

Fingerprint

Streptococcus mutans
Plant Extracts
Acids
Seeds
Fruit
Vitis
Tea
Gels
Starch
Propolis
Glycyrrhiza
Ribes
Financial Support
Microelectrodes
Finland
Zea mays
Mouth
In Vitro Techniques
Suspensions
Oils

Cite this

Söderling, E. ; Karjalainen, S. ; Lille, Martina ; Nohynek, Liisa ; Autio, Karin ; Saarela, Maria. / Effects of various plant extracts on acid production by plaque and mutans streptococci. In: Caries Research. 2004 ; Vol. 38. pp. 371.
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title = "Effects of various plant extracts on acid production by plaque and mutans streptococci",
abstract = "The aim of this study was to compare the ability of selected plant extracts to inhibit acid production from starch gels by plaque in vivo and by mutans streptococci in vitro. Commercially available propolis, liquorice, green tea, grape fruit seed, black currant seed and cloudberry seed extracts were used in the study. The extracts were added to a starch gel, which consisted of 8{\%} acid hydrolysed corn starch, 25{\%} maltitol syrup and water (w/w). The pH of the gel was adjusted close to 6,5. For the in vivo acid production tests the subjects accumulated plaque for 2-3 days. After a single intake of 6 g of the test product, it was swished around in the mouth for 2 min. Changes of plaque pH in 2-3 interdental spaces were measured with a microelectrode (Beetrode) for 30 min. In the in vitro tests Streptococcus mutans NCTC 10449 was mixed with the gel suspensions and acid production was followed electrometrically for up to 10 min. Propolis inhibited acid production both in vitro and in vivo at ≥ 5{\%}. Liquorice inhibited acid production at  1{\%} in vitro and at  5.0 {\%} in vivo. Grape fruit seed and green tea extracts prevented acid production completely: grape fruit seed in vitro at  0.5{\%} and green tea in vitro at  0.1{\%} and in vivo at 1.0 {\%}. Berry seed oil extracts (1 {\%}) had no effect on acid production. In conclusion, acid production was inhibited by several plant extracts, which makes them promising components of toothfriendly products. Financial support by the National Technology Agency (Tekes), Finland, is gratefully acknowledged.",
author = "E. S{\"o}derling and S. Karjalainen and Martina Lille and Liisa Nohynek and Karin Autio and Maria Saarela",
year = "2004",
language = "English",
volume = "38",
pages = "371",
journal = "Caries Research",
issn = "0008-6568",
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}

Söderling, E, Karjalainen, S, Lille, M, Nohynek, L, Autio, K & Saarela, M 2004, 'Effects of various plant extracts on acid production by plaque and mutans streptococci', Caries Research, vol. 38, pp. 371.

Effects of various plant extracts on acid production by plaque and mutans streptococci. / Söderling, E.; Karjalainen, S.; Lille, Martina; Nohynek, Liisa; Autio, Karin; Saarela, Maria.

In: Caries Research, Vol. 38, 2004, p. 371.

Research output: Contribution to journalOther journal contributionScientific

TY - JOUR

T1 - Effects of various plant extracts on acid production by plaque and mutans streptococci

AU - Söderling, E.

AU - Karjalainen, S.

AU - Lille, Martina

AU - Nohynek, Liisa

AU - Autio, Karin

AU - Saarela, Maria

PY - 2004

Y1 - 2004

N2 - The aim of this study was to compare the ability of selected plant extracts to inhibit acid production from starch gels by plaque in vivo and by mutans streptococci in vitro. Commercially available propolis, liquorice, green tea, grape fruit seed, black currant seed and cloudberry seed extracts were used in the study. The extracts were added to a starch gel, which consisted of 8% acid hydrolysed corn starch, 25% maltitol syrup and water (w/w). The pH of the gel was adjusted close to 6,5. For the in vivo acid production tests the subjects accumulated plaque for 2-3 days. After a single intake of 6 g of the test product, it was swished around in the mouth for 2 min. Changes of plaque pH in 2-3 interdental spaces were measured with a microelectrode (Beetrode) for 30 min. In the in vitro tests Streptococcus mutans NCTC 10449 was mixed with the gel suspensions and acid production was followed electrometrically for up to 10 min. Propolis inhibited acid production both in vitro and in vivo at ≥ 5%. Liquorice inhibited acid production at  1% in vitro and at  5.0 % in vivo. Grape fruit seed and green tea extracts prevented acid production completely: grape fruit seed in vitro at  0.5% and green tea in vitro at  0.1% and in vivo at 1.0 %. Berry seed oil extracts (1 %) had no effect on acid production. In conclusion, acid production was inhibited by several plant extracts, which makes them promising components of toothfriendly products. Financial support by the National Technology Agency (Tekes), Finland, is gratefully acknowledged.

AB - The aim of this study was to compare the ability of selected plant extracts to inhibit acid production from starch gels by plaque in vivo and by mutans streptococci in vitro. Commercially available propolis, liquorice, green tea, grape fruit seed, black currant seed and cloudberry seed extracts were used in the study. The extracts were added to a starch gel, which consisted of 8% acid hydrolysed corn starch, 25% maltitol syrup and water (w/w). The pH of the gel was adjusted close to 6,5. For the in vivo acid production tests the subjects accumulated plaque for 2-3 days. After a single intake of 6 g of the test product, it was swished around in the mouth for 2 min. Changes of plaque pH in 2-3 interdental spaces were measured with a microelectrode (Beetrode) for 30 min. In the in vitro tests Streptococcus mutans NCTC 10449 was mixed with the gel suspensions and acid production was followed electrometrically for up to 10 min. Propolis inhibited acid production both in vitro and in vivo at ≥ 5%. Liquorice inhibited acid production at  1% in vitro and at  5.0 % in vivo. Grape fruit seed and green tea extracts prevented acid production completely: grape fruit seed in vitro at  0.5% and green tea in vitro at  0.1% and in vivo at 1.0 %. Berry seed oil extracts (1 %) had no effect on acid production. In conclusion, acid production was inhibited by several plant extracts, which makes them promising components of toothfriendly products. Financial support by the National Technology Agency (Tekes), Finland, is gratefully acknowledged.

M3 - Other journal contribution

VL - 38

SP - 371

JO - Caries Research

JF - Caries Research

SN - 0008-6568

ER -