Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs

Gun Wirtanen, Mervi Aalto, Päivi Härkönen, Peter Gilbert, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

This paper describes the evaluation of poloxamer-hydrogel biofilm-constructs for the routine efficacy testing of disinfectants at normal use strength. Aqueous solutions of poloxamer Pluronic F127 show thermo-reversible gelation, being liquid at temperatures <15 °C but firm gels at temperatures >15 °C. Chilled poloxamer solutions (30% w/v) were made up in a tryptone soy broth and inoculated with stationary-phase cultures of 14 foodborne spoilage microbes, including Pseudomonas, Bacillus, Staphylococcus, Micrococcus, enterobacteria and a yeast, as well as pathogen test-strains, including Listeria and Salmonella. Drops (either 200 µl or 100 µl) were placed onto pre-warmed, sterile, stainless steel discs held in sealed Petri dishes. The constructs were incubated for 5 h at 30 °C and all strains grew well in the poloxamer hydrogel. Incubated poloxamer gels and their discs were transferred to solutions of commercial disinfectant formulations containing either amphoteric surfactants, hydrogen peroxide with peracetic acid or silver ions, sodium hypochlorite, or alcohols with and without additives. After 5 min at 25 °C the test pieces were removed from the disinfectant solution and transferred to a neutraliser at 10–15 °C. These tests were carried out in triplicate. The gels dispersed rapidly, releasing the cells and enabling a count of the viable cells. All formulations effected a >5-log kill of planktonic challenges within 5 min. An effective killing of microbial cells within the biofilm-constructs was shown when the reduction was at least 0.3 log units. The results were highly reproducible, with patterns of susceptibility varying as a function of the organism, disinfectant type, and concentration. The experiments support the view that poloxamer hydrogels can be used for testing the disinfectant efficacy of various formulations against contaminants isolated from food and drink processes.
Original languageEnglish
Pages (from-to)409-414
Number of pages6
JournalEuropean Food Research and Technology
Volume213
Issue number4-5
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Spoilage
Disinfectants
Poloxamer
disinfectants
Biofilms
spoilage
biofilm
hydrocolloids
microorganisms
Hydrogels
Testing
testing
UCON 50-HB-5100
Hydrogel
Amphoteric surfactants
Gels
gels
peracetic acid
Listeria
Micrococcus

Cite this

Wirtanen, Gun ; Aalto, Mervi ; Härkönen, Päivi ; Gilbert, Peter ; Mattila-Sandholm, Tiina. / Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs. In: European Food Research and Technology. 2001 ; Vol. 213, No. 4-5. pp. 409-414.
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abstract = "This paper describes the evaluation of poloxamer-hydrogel biofilm-constructs for the routine efficacy testing of disinfectants at normal use strength. Aqueous solutions of poloxamer Pluronic F127 show thermo-reversible gelation, being liquid at temperatures <15 °C but firm gels at temperatures >15 °C. Chilled poloxamer solutions (30{\%} w/v) were made up in a tryptone soy broth and inoculated with stationary-phase cultures of 14 foodborne spoilage microbes, including Pseudomonas, Bacillus, Staphylococcus, Micrococcus, enterobacteria and a yeast, as well as pathogen test-strains, including Listeria and Salmonella. Drops (either 200 µl or 100 µl) were placed onto pre-warmed, sterile, stainless steel discs held in sealed Petri dishes. The constructs were incubated for 5 h at 30 °C and all strains grew well in the poloxamer hydrogel. Incubated poloxamer gels and their discs were transferred to solutions of commercial disinfectant formulations containing either amphoteric surfactants, hydrogen peroxide with peracetic acid or silver ions, sodium hypochlorite, or alcohols with and without additives. After 5 min at 25 °C the test pieces were removed from the disinfectant solution and transferred to a neutraliser at 10–15 °C. These tests were carried out in triplicate. The gels dispersed rapidly, releasing the cells and enabling a count of the viable cells. All formulations effected a >5-log kill of planktonic challenges within 5 min. An effective killing of microbial cells within the biofilm-constructs was shown when the reduction was at least 0.3 log units. The results were highly reproducible, with patterns of susceptibility varying as a function of the organism, disinfectant type, and concentration. The experiments support the view that poloxamer hydrogels can be used for testing the disinfectant efficacy of various formulations against contaminants isolated from food and drink processes.",
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Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs. / Wirtanen, Gun; Aalto, Mervi; Härkönen, Päivi; Gilbert, Peter; Mattila-Sandholm, Tiina.

In: European Food Research and Technology, Vol. 213, No. 4-5, 2001, p. 409-414.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs

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AU - Aalto, Mervi

AU - Härkönen, Päivi

AU - Gilbert, Peter

AU - Mattila-Sandholm, Tiina

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N2 - This paper describes the evaluation of poloxamer-hydrogel biofilm-constructs for the routine efficacy testing of disinfectants at normal use strength. Aqueous solutions of poloxamer Pluronic F127 show thermo-reversible gelation, being liquid at temperatures <15 °C but firm gels at temperatures >15 °C. Chilled poloxamer solutions (30% w/v) were made up in a tryptone soy broth and inoculated with stationary-phase cultures of 14 foodborne spoilage microbes, including Pseudomonas, Bacillus, Staphylococcus, Micrococcus, enterobacteria and a yeast, as well as pathogen test-strains, including Listeria and Salmonella. Drops (either 200 µl or 100 µl) were placed onto pre-warmed, sterile, stainless steel discs held in sealed Petri dishes. The constructs were incubated for 5 h at 30 °C and all strains grew well in the poloxamer hydrogel. Incubated poloxamer gels and their discs were transferred to solutions of commercial disinfectant formulations containing either amphoteric surfactants, hydrogen peroxide with peracetic acid or silver ions, sodium hypochlorite, or alcohols with and without additives. After 5 min at 25 °C the test pieces were removed from the disinfectant solution and transferred to a neutraliser at 10–15 °C. These tests were carried out in triplicate. The gels dispersed rapidly, releasing the cells and enabling a count of the viable cells. All formulations effected a >5-log kill of planktonic challenges within 5 min. An effective killing of microbial cells within the biofilm-constructs was shown when the reduction was at least 0.3 log units. The results were highly reproducible, with patterns of susceptibility varying as a function of the organism, disinfectant type, and concentration. The experiments support the view that poloxamer hydrogels can be used for testing the disinfectant efficacy of various formulations against contaminants isolated from food and drink processes.

AB - This paper describes the evaluation of poloxamer-hydrogel biofilm-constructs for the routine efficacy testing of disinfectants at normal use strength. Aqueous solutions of poloxamer Pluronic F127 show thermo-reversible gelation, being liquid at temperatures <15 °C but firm gels at temperatures >15 °C. Chilled poloxamer solutions (30% w/v) were made up in a tryptone soy broth and inoculated with stationary-phase cultures of 14 foodborne spoilage microbes, including Pseudomonas, Bacillus, Staphylococcus, Micrococcus, enterobacteria and a yeast, as well as pathogen test-strains, including Listeria and Salmonella. Drops (either 200 µl or 100 µl) were placed onto pre-warmed, sterile, stainless steel discs held in sealed Petri dishes. The constructs were incubated for 5 h at 30 °C and all strains grew well in the poloxamer hydrogel. Incubated poloxamer gels and their discs were transferred to solutions of commercial disinfectant formulations containing either amphoteric surfactants, hydrogen peroxide with peracetic acid or silver ions, sodium hypochlorite, or alcohols with and without additives. After 5 min at 25 °C the test pieces were removed from the disinfectant solution and transferred to a neutraliser at 10–15 °C. These tests were carried out in triplicate. The gels dispersed rapidly, releasing the cells and enabling a count of the viable cells. All formulations effected a >5-log kill of planktonic challenges within 5 min. An effective killing of microbial cells within the biofilm-constructs was shown when the reduction was at least 0.3 log units. The results were highly reproducible, with patterns of susceptibility varying as a function of the organism, disinfectant type, and concentration. The experiments support the view that poloxamer hydrogels can be used for testing the disinfectant efficacy of various formulations against contaminants isolated from food and drink processes.

U2 - 10.1007/s002170100375

DO - 10.1007/s002170100375

M3 - Article

VL - 213

SP - 409

EP - 414

JO - European Food Research and Technology

JF - European Food Research and Technology

SN - 1438-2377

IS - 4-5

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