Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments

Tarja Nevanen (Corresponding Author), L. Söderholm, K. Kukkonen, Tapani Suortti, Tuija Teerinen, Markus Linder, Hans Söderlund, Tuula Teeri

    Research output: Contribution to journalArticleScientificpeer-review

    49 Citations (Scopus)

    Abstract

    There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process.
    Original languageEnglish
    Pages (from-to)89-97
    Number of pages9
    JournalJournal of Chromatography A
    Volume925
    Issue number1-2
    DOIs
    Publication statusPublished - 2001
    MoE publication typeA1 Journal article-refereed

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