Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments

Tarja Nevanen (Corresponding Author), L. Söderholm, K. Kukkonen, Tapani Suortti, Tuija Teerinen, Markus Linder, Hans Söderlund, Tuula Teeri

Research output: Contribution to journalArticleScientificpeer-review

49 Citations (Scopus)

Abstract

There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process.
Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalJournal of Chromatography A
Volume925
Issue number1-2
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Immobilized Antibodies
Immunoglobulin Fragments
Enantiomers
Antibodies
Pharmaceutical Preparations
Genetic engineering
Genetic Engineering
Antibody Specificity
Molecular mass
Purification
Molecules

Cite this

Nevanen, Tarja ; Söderholm, L. ; Kukkonen, K. ; Suortti, Tapani ; Teerinen, Tuija ; Linder, Markus ; Söderlund, Hans ; Teeri, Tuula. / Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments. In: Journal of Chromatography A. 2001 ; Vol. 925, No. 1-2. pp. 89-97.
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Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments. / Nevanen, Tarja (Corresponding Author); Söderholm, L.; Kukkonen, K.; Suortti, Tapani; Teerinen, Tuija; Linder, Markus; Söderlund, Hans; Teeri, Tuula.

In: Journal of Chromatography A, Vol. 925, No. 1-2, 2001, p. 89-97.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Efficient enantioselective separation of drug enantiomers by immobilized antibody fragments

AU - Nevanen, Tarja

AU - Söderholm, L.

AU - Kukkonen, K.

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AU - Teerinen, Tuija

AU - Linder, Markus

AU - Söderlund, Hans

AU - Teeri, Tuula

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AB - There is an increasing need for methods for efficient enantioselective separation and purification of chiral drugs. Genetic engineering provides the means for generating recombinant antibodies exhibiting extremely high specificity for even small molecular mass compounds. Here, recombinant antibody fragments have been generated for the drug diarylalkyltriazole that contains two chiral centres. Immobilised antibody fragments has been used successfully for efficient, step-wise separation of two enantiomers of the drug. Owing to the antibody specificity, one enantiomer came out in the flow-through, while the bound enantiomer could be specifically eluted. One of the antibodies tolerated solvents required both for dissolving the target molecules and for their elution for extended times and was shown to function over multiple cycles of the separation process.

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