Abstract
Successful plant regeneration was achieved for the first time from hairy root-derived protoplasts of Hyoscyamus muticus. High yields (7 × 106 / g fresh weight) of protoplasts were isolated directly from the transformed roots of Hyoscyamus muticus using an enzyme mixture comprising 1 % macerozyme and 2 % cellulase in an osmoticum consisting of 0.2 M CaCl2 and 0.6 M mannitol. Protoplasts were first cultured in liquid NT/PRO I medium and further on semi-solid NT/PRO II agar medium. The procedure permits highly efficient formation of colonies. The plating efficiency varied from 1-9 %. The small individual colonies regenerated easily into shoots and roots at frequencies of 18 % and 70 %, respectively. The time required for the development of small plantlets from protoplasts was 8-11 weeks. The regenerated plants contained rolB from Ri-T-DNA and exhibited an altered phenotype compared to the control plants.
Original language | English |
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Pages (from-to) | 738-742 |
Number of pages | 5 |
Journal | Plant Cell Reports |
Volume | 14 |
Issue number | 11 |
DOIs | |
Publication status | Published - 1 Aug 1995 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Hyoscyamus muticus
- plant regeneration
- protoplasts
- transformed roots