Efficient production of antibody fragments by the filamentous fungus trichoderma reesei

Eini Nyyssönen; Merja Penttilä; Anu Harkki; Anu Saloheimo; Jonathan K C Knowles; Sirkka Keränen

doi:10.1038/nbt0593-591

Efficient production of antibody fragments by the filamentous fungus trichoderma reesei

Eini Nyyssönen, Merja Penttilä, Anu Harkki, Anu Saloheimo, Jonathan K C Knowles, Sirkka Keränen^*

^*Corresponding author for this work

Glaxo Institute for Molecular Biology

Research output: Contribution to journal › Article › Scientific › peer-review

104 Citations (Scopus)

Abstract

We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.

Original language	English
Pages (from-to)	591-595
Number of pages	5
Journal	Bio/Technology
Volume	11
Issue number	5
DOIs	https://doi.org/10.1038/nbt0593-591
Publication status	Published - 1 Jan 1993
MoE publication type	A1 Journal article-refereed

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abstract = "We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.",

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AU - Penttilä, Merja

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AU - Saloheimo, Anu

AU - Knowles, Jonathan K C

AU - Keränen, Sirkka

PY - 1993/1/1

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Efficient production of antibody fragments by the filamentous fungus trichoderma reesei

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