Efficient production of antibody fragments by the filamentous fungus trichoderma reesei

Eini Nyyssönen, Merja Penttilä, Anu Harkki, Anu Saloheimo, Jonathan K C Knowles, Sirkka Keränen

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Abstract

We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.

Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalBio/Technology
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Jan 1993
MoE publication typeA1 Journal article-refereed

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    Nyyssönen, E., Penttilä, M., Harkki, A., Saloheimo, A., Knowles, J. K. C., & Keränen, S. (1993). Efficient production of antibody fragments by the filamentous fungus trichoderma reesei. Bio/Technology, 11(5), 591-595. https://doi.org/10.1038/nbt0593-591