Efficient production of antibody fragments by the filamentous fungus trichoderma reesei

Eini Nyyssönen, Merja Penttilä, Anu Harkki, Anu Saloheimo, Jonathan K C Knowles, Sirkka Keränen

Research output: Contribution to journalArticleScientificpeer-review

94 Citations (Scopus)

Abstract

We have engineered the filamentous fungus Trichoderma reesei to assemble and secrete immunologically authentic engineered Fab antibody fragments into the culture medium. A major improvement in yield was achieved by fusing the heavy Fd chain to the T. reesei cellulase, CBHI. The yields of secreted, immunologically active Fab and CBHI-Fab fusion were 1 mg/1 and 150 mg/1, respectively. The Fab fragment can be released from the fusion protein CBHI-Fab by an extracellular T. reesei protease. There was no detectable difference in affinity for the antigen between the engineered Fab and the idiotypic antibody.

Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalBio/Technology
Volume11
Issue number5
DOIs
Publication statusPublished - 1 Jan 1993
MoE publication typeA1 Journal article-refereed

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Immunoglobulin Fragments
Immunoglobulin Fab Fragments
Trichoderma
Fungi
Antibodies
Fusion reactions
Cellulase
Antigens
Culture Media
Peptide Hydrolases
Proteins

Cite this

Nyyssönen, Eini ; Penttilä, Merja ; Harkki, Anu ; Saloheimo, Anu ; Knowles, Jonathan K C ; Keränen, Sirkka. / Efficient production of antibody fragments by the filamentous fungus trichoderma reesei. In: Bio/Technology. 1993 ; Vol. 11, No. 5. pp. 591-595.
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Efficient production of antibody fragments by the filamentous fungus trichoderma reesei. / Nyyssönen, Eini; Penttilä, Merja; Harkki, Anu; Saloheimo, Anu; Knowles, Jonathan K C; Keränen, Sirkka.

In: Bio/Technology, Vol. 11, No. 5, 01.01.1993, p. 591-595.

Research output: Contribution to journalArticleScientificpeer-review

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