Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis

Marja Ilmén, Kari Koivuranta, Laura Ruohonen, Pirkko Suominen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.
Original languageEnglish
Title of host publicationInternational Specialised Symposium on Yeasts ISSY25
Subtitle of host publicationSystems Biology of Yeasts - from Models to Applications
EditorsAnnemari Kuokka, Merja Penttilä
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages150
ISBN (Electronic)951-38-6308-5
ISBN (Print)951-38-6307-7
Publication statusPublished - 2006
EventInternational Specialised Symposium on Yeasts, ISSY 25 - Espoo, Finland
Duration: 18 Jun 200621 Jun 2006

Publication series

SeriesVTT Symposium
Number242
ISSN0357-9387

Conference

ConferenceInternational Specialised Symposium on Yeasts, ISSY 25
Abbreviated titleISSY 25
CountryFinland
CityEspoo
Period18/06/0621/06/06

Fingerprint

Scheffersomyces stipitis
xylose
lactic acid
lactates
yeasts
glucose
lactate dehydrogenase
ethanol
Lactobacillus helveticus
ethanol production
promoter regions
oxygen

Cite this

Ilmén, M., Koivuranta, K., Ruohonen, L., Suominen, P., & Penttilä, M. (2006). Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. In A. Kuokka, & M. Penttilä (Eds.), International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications (pp. 150). [P94] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 242
Ilmén, Marja ; Koivuranta, Kari ; Ruohonen, Laura ; Suominen, Pirkko ; Penttilä, Merja. / Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. editor / Annemari Kuokka ; Merja Penttilä. Espoo : VTT Technical Research Centre of Finland, 2006. pp. 150 (VTT Symposium; No. 242).
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Ilmén, M, Koivuranta, K, Ruohonen, L, Suominen, P & Penttilä, M 2006, Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. in A Kuokka & M Penttilä (eds), International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications., P94, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 242, pp. 150, International Specialised Symposium on Yeasts, ISSY 25 , Espoo, Finland, 18/06/06.

Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. / Ilmén, Marja; Koivuranta, Kari; Ruohonen, Laura; Suominen, Pirkko; Penttilä, Merja.

International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. ed. / Annemari Kuokka; Merja Penttilä. Espoo : VTT Technical Research Centre of Finland, 2006. p. 150 P94 (VTT Symposium; No. 242).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis

AU - Ilmén, Marja

AU - Koivuranta, Kari

AU - Ruohonen, Laura

AU - Suominen, Pirkko

AU - Penttilä, Merja

PY - 2006

Y1 - 2006

N2 - Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.

AB - Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.

M3 - Conference abstract in proceedings

SN - 951-38-6307-7

T3 - VTT Symposium

SP - 150

BT - International Specialised Symposium on Yeasts ISSY25

A2 - Kuokka, Annemari

A2 - Penttilä, Merja

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Ilmén M, Koivuranta K, Ruohonen L, Suominen P, Penttilä M. Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. In Kuokka A, Penttilä M, editors, International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications. Espoo: VTT Technical Research Centre of Finland. 2006. p. 150. P94. (VTT Symposium; No. 242).