Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis

Marja Ilmén, Kari Koivuranta, Laura Ruohonen, Pirkko Suominen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.
Original languageEnglish
Title of host publicationInternational Specialised Symposium on Yeasts ISSY25
Subtitle of host publicationSystems Biology of Yeasts - from Models to Applications
EditorsAnnemari Kuokka, Merja Penttilä
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages150
ISBN (Electronic)951-38-6308-5
ISBN (Print)951-38-6307-7
Publication statusPublished - 2006
EventInternational Specialised Symposium on Yeasts, ISSY 25 - Espoo, Finland
Duration: 18 Jun 200621 Jun 2006

Publication series

SeriesVTT Symposium
Number242
ISSN0357-9387

Conference

ConferenceInternational Specialised Symposium on Yeasts, ISSY 25
Abbreviated titleISSY 25
CountryFinland
CityEspoo
Period18/06/0621/06/06

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    Ilmén, M., Koivuranta, K., Ruohonen, L., Suominen, P., & Penttilä, M. (2006). Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. In A. Kuokka, & M. Penttilä (Eds.), International Specialised Symposium on Yeasts ISSY25: Systems Biology of Yeasts - from Models to Applications (pp. 150). [P94] VTT Technical Research Centre of Finland. VTT Symposium, No. 242 http://www.vtt.fi/inf/pdf/symposiums/2006/S242.pdf