Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis

Marja Ilmen (Corresponding author), Kari Koivuranta, Laura Ruohonen, Pirkko Suominen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3
EditorsAnnemari Kuokka-Ihalainen, Markku Saloheimo, Tiina Pakula
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages159
ISBN (Electronic) 978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

NameVTT Symposium
PublisherVTT
Volume245
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

Fingerprint

Scheffersomyces stipitis
xylose
lactic acid
lactates
yeasts
glucose
lactate dehydrogenase
ethanol
Lactobacillus helveticus
ethanol production
promoter regions
oxygen

Cite this

Ilmen, M., Koivuranta, K., Ruohonen, L., Suominen, P., & Penttilä, M. (2007). Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. In A. Kuokka-Ihalainen, M. Saloheimo, & T. Pakula (Eds.), 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 159). [P101] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, Vol.. 245
Ilmen, Marja ; Koivuranta, Kari ; Ruohonen, Laura ; Suominen, Pirkko ; Penttilä, Merja. / Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. editor / Annemari Kuokka-Ihalainen ; Markku Saloheimo ; Tiina Pakula. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 159 (VTT Symposium, Vol. 245).
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abstract = "Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20{\%} of the wild type levels on xylose-containing medium and to 75{\%} on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.",
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Ilmen, M, Koivuranta, K, Ruohonen, L, Suominen, P & Penttilä, M 2007, Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. in A Kuokka-Ihalainen, M Saloheimo & T Pakula (eds), 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3., P101, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, vol. 245, pp. 159, 3rd European Federation of Biotechnology Conference , Helsinki, Finland, 13/06/07.

Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. / Ilmen, Marja (Corresponding author); Koivuranta, Kari; Ruohonen, Laura; Suominen, Pirkko; Penttilä, Merja.

3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. ed. / Annemari Kuokka-Ihalainen; Markku Saloheimo; Tiina Pakula. Espoo : VTT Technical Research Centre of Finland, 2007. p. 159 P101 (VTT Symposium, Vol. 245).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis

AU - Ilmen, Marja

AU - Koivuranta, Kari

AU - Ruohonen, Laura

AU - Suominen, Pirkko

AU - Penttilä, Merja

PY - 2007

Y1 - 2007

N2 - Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.

AB - Pichia stipitis, a yeast which ferments naturally xylose, was genetically engineered for L-(+)-lactate production. A P. stipitis strain expressing the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter was constructed. Either xylose or glucose was used as the carbon source for lactate production under oxygen restricted conditions. Remarkably, the constructed P. stipitis strain produced a higher lactate concentration and yield on xylose than on glucose. Lactate accumulated as the main product on xylose-containing medium: 58 g/l lactate was produced from 100 g/l xylose. Relatively efficient lactate production was also observed on glucose medium, 41 g/l lactate was produced at a yield of 0.44 g/g glucose. Lactate was produced at the expense of ethanol production which was decreased to approximately 20% of the wild type levels on xylose-containing medium and to 75% on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact.

M3 - Conference abstract in proceedings

SN - 978-951-38-6313-5

T3 - VTT Symposium

SP - 159

BT - 3rd European Federation of Biotechnology Conference

A2 - Kuokka-Ihalainen, Annemari

A2 - Saloheimo, Markku

A2 - Pakula, Tiina

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Ilmen M, Koivuranta K, Ruohonen L, Suominen P, Penttilä M. Efficient production of L-lactic acid from xylose by metabolically engineered yeast Pichia stipitis. In Kuokka-Ihalainen A, Saloheimo M, Pakula T, editors, 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo: VTT Technical Research Centre of Finland. 2007. p. 159. P101. (VTT Symposium, Vol. 245).