Efficient production of L-lactic acid from xylose by Pichia stipitis

Marja Ilmén, Kari Koivuranta, Laura Ruohonen, Pirkko Suominen, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

89 Citations (Scopus)

Abstract

Microbial conversion of renewable raw materials to useful products is an important objective in industrial biotechnology. Pichia stipitis, a yeast that naturally ferments xylose, was genetically engineered for L-(+)-lactate production. We constructed a P. stipitis strain that expressed the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter. Xylose, glucose, or a mixture of the two sugars was used as the carbon source for lactate production. The constructed P. stipitis strain produced a higher level of lactate and a higher yield on xylose than on glucose. Lactate accumulated as the main product in xylose-containing medium, with 58 g/liter lactate produced from 100 g/liter xylose. Relatively efficient lactate production also occurred on glucose medium, with 41 g/liter lactate produced from 94 g/liter glucose. In the presence of both sugars, xylose and glucose were consumed simultaneously and converted predominantly to lactate. Lactate was produced at the expense of ethanol, whose production decreased to ∼15 to 30% of the wild-type level on xylose-containing medium and to 70 to 80% of the wild-type level on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact. Our results show, for the first time, that lactate production from xylose by a yeast species is feasible and efficient. This is encouraging for further development of yeast-based bioprocesses to produce lactate from lignocellulosic raw material.

Original languageEnglish
Pages (from-to)117-123
JournalApplied and Environmental Microbiology
Volume73
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007
MoE publication typeA1 Journal article-refereed

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Scheffersomyces stipitis
Pichia
Xylose
xylose
lactic acid
lactates
Lactic Acid
glucose
acid
yeast
ethanol
sugar
Ethanol
Yeasts
yeasts
lactate dehydrogenase
Pyruvic Acid
L-Lactate Dehydrogenase
Glucose
biotechnology

Cite this

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title = "Efficient production of L-lactic acid from xylose by Pichia stipitis",
abstract = "Microbial conversion of renewable raw materials to useful products is an important objective in industrial biotechnology. Pichia stipitis, a yeast that naturally ferments xylose, was genetically engineered for L-(+)-lactate production. We constructed a P. stipitis strain that expressed the L-lactate dehydrogenase (LDH) from Lactobacillus helveticus under the control of the P. stipitis fermentative ADH1 promoter. Xylose, glucose, or a mixture of the two sugars was used as the carbon source for lactate production. The constructed P. stipitis strain produced a higher level of lactate and a higher yield on xylose than on glucose. Lactate accumulated as the main product in xylose-containing medium, with 58 g/liter lactate produced from 100 g/liter xylose. Relatively efficient lactate production also occurred on glucose medium, with 41 g/liter lactate produced from 94 g/liter glucose. In the presence of both sugars, xylose and glucose were consumed simultaneously and converted predominantly to lactate. Lactate was produced at the expense of ethanol, whose production decreased to ∼15 to 30{\%} of the wild-type level on xylose-containing medium and to 70 to 80{\%} of the wild-type level on glucose-containing medium. Thus, LDH competed efficiently with the ethanol pathway for pyruvate, even though the pathway from pyruvate to ethanol was intact. Our results show, for the first time, that lactate production from xylose by a yeast species is feasible and efficient. This is encouraging for further development of yeast-based bioprocesses to produce lactate from lignocellulosic raw material.",
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Efficient production of L-lactic acid from xylose by Pichia stipitis. / Ilmén, Marja; Koivuranta, Kari; Ruohonen, Laura; Suominen, Pirkko; Penttilä, Merja.

In: Applied and Environmental Microbiology, Vol. 73, No. 1, 01.01.2007, p. 117-123.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Efficient production of L-lactic acid from xylose by Pichia stipitis

AU - Ilmén, Marja

AU - Koivuranta, Kari

AU - Ruohonen, Laura

AU - Suominen, Pirkko

AU - Penttilä, Merja

N1 - HUO: NT Food Solutions CA2: TK402 ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY

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