Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.)

Marjatta Salmenkallio, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002% to 0.015%. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.
Original languageEnglish
Pages (from-to)253-256
JournalPlant Cell Reports
Volume14
Issue number4
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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microspores
Hordeum vulgare
protoplasts
barley
malting barley
agarose
plantlets
inflorescences
cultivars
seeds
soil

Cite this

@article{cc393ad2e53046d59e11504430185dd6,
title = "Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.)",
abstract = "Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002{\%} to 0.015{\%}. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.",
author = "Marjatta Salmenkallio and Veli Kauppinen",
note = "Project code: BEL3030",
year = "1995",
doi = "10.1007/BF00233644",
language = "English",
volume = "14",
pages = "253--256",
journal = "Plant Cell Reports",
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Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.). / Salmenkallio, Marjatta; Kauppinen, Veli.

In: Plant Cell Reports, Vol. 14, No. 4, 1995, p. 253-256.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.)

AU - Salmenkallio, Marjatta

AU - Kauppinen, Veli

N1 - Project code: BEL3030

PY - 1995

Y1 - 1995

N2 - Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002% to 0.015%. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.

AB - Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002% to 0.015%. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.

U2 - 10.1007/BF00233644

DO - 10.1007/BF00233644

M3 - Article

VL - 14

SP - 253

EP - 256

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 4

ER -