Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae

Merja E. Penttilä, Lars André, Päivi Lehtovaara, Michael Bailey, Tuula T. Teeri, Jonathan K.C. Knowles

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Abstract

Two different cellobiohydrolases, CBHI and CBHII, of the filamentous fungus Trichoderma reesei both hydrolyse highly crystalline cellulose. Cellulolytic strains of the yeast Saccharomyces cerevisiae were constructed by transferring cDNAs coding for these enzymes into yeast on an expression plasmid. These cellulolytic yeasts were able to secrete efficiently the large, heterologous proteins to the culture medium. The recombinant cellulases were observed to be heterogeneous in Mr due, at least partly, to variable N-glycosylation. Recombinant CBHII was able to bind to crystalline cellulose, although slightly less efficiently than the native enzyme. Both of the two recombinant cellulases were able to degrade amorphous cellulose. In a fermenter cultivation, around 100 μg/ml of CBHII was secreted into the yeast growth medium.

Original languageEnglish
Pages (from-to)103-112
Number of pages1
JournalGene
Volume63
Issue number1
DOIs
Publication statusPublished - 15 Mar 1988
MoE publication typeA1 Journal article-refereed

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Keywords

  • cellulases
  • cellulolytic yeast
  • glycosylation
  • Recombinant DNA

Cite this

Penttilä, M. E., André, L., Lehtovaara, P., Bailey, M., Teeri, T. T., & Knowles, J. K. C. (1988). Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae. Gene, 63(1), 103-112. https://doi.org/10.1016/0378-1119(88)90549-5