TY - JOUR
T1 - Efficient strategy to alleviate the inhibitory effect of lignin-derived compounds for enhanced butanol production
AU - Survase, Shrikant A.
AU - Nimbalkar, Pranhita
AU - Jurgens, German
AU - Granström, Tom
AU - Chavan, Prakash
AU - Bankar, Sandip Balasaheb
N1 - Funding Information:
This study belongs to the project of Commodity Chemicals from Forest Biomass-Bioforest, which is part of the Biorefine Technology program financed by Tekes (The Finnish Funding Agency for Technology and Innovation, Finland). In addition, financial support from industrial collaborators is greatly acknowledged.
Publisher Copyright:
© 2020 American Chemical Society.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/25
Y1 - 2021/1/25
N2 - In the present study, the effect of one of the most important lignin-derived inhibitors (lignosulfonate) was assessed. A technique to overcome the lignosulfonate inhibitory action in the acetone-butanol-ethanol (ABE) fermentation process is proposed here. Different lignosulfonates were primarily added in the fermentation medium to observe their mechanistic action on the ABE production profile. Augmenting lignosulfonate concentration (>0.5 g L-1) resulted in a drastically reduced solvent titer (ABE a1.50 g L-1). Especially, low-molecular-weight lignosulfonate (>1 g L-1) severely affected the solvent production and completely ceased the fermentation process. Therefore, a strategic approach that triggers the key genes responsible for butanol production was explored. The experimental analysis revealed that soy meal addition could enhance Clostridium acetobutylicum survival in the presence of lignosulfonates (0.25-3 g L-1). Moreover, soy meal addition also enhanced butanol concentration over 1.5-fold as compared to the control experiment. The ABE production using wood hydrolysate also produced substantial solvent titer (ABE a11 g L-1) in the presence of soy meal (5 g L-1). The transcriptional analysis results showed that important genes in clostridial metabolic pathways were upregulated in the presence of soy meal addition during fermentation.
AB - In the present study, the effect of one of the most important lignin-derived inhibitors (lignosulfonate) was assessed. A technique to overcome the lignosulfonate inhibitory action in the acetone-butanol-ethanol (ABE) fermentation process is proposed here. Different lignosulfonates were primarily added in the fermentation medium to observe their mechanistic action on the ABE production profile. Augmenting lignosulfonate concentration (>0.5 g L-1) resulted in a drastically reduced solvent titer (ABE a1.50 g L-1). Especially, low-molecular-weight lignosulfonate (>1 g L-1) severely affected the solvent production and completely ceased the fermentation process. Therefore, a strategic approach that triggers the key genes responsible for butanol production was explored. The experimental analysis revealed that soy meal addition could enhance Clostridium acetobutylicum survival in the presence of lignosulfonates (0.25-3 g L-1). Moreover, soy meal addition also enhanced butanol concentration over 1.5-fold as compared to the control experiment. The ABE production using wood hydrolysate also produced substantial solvent titer (ABE a11 g L-1) in the presence of soy meal (5 g L-1). The transcriptional analysis results showed that important genes in clostridial metabolic pathways were upregulated in the presence of soy meal addition during fermentation.
UR - http://www.scopus.com/inward/record.url?scp=85099135416&partnerID=8YFLogxK
U2 - 10.1021/acssuschemeng.0c06584
DO - 10.1021/acssuschemeng.0c06584
M3 - Article
AN - SCOPUS:85099135416
SN - 2168-0485
VL - 9
SP - 1172
EP - 1179
JO - ACS Sustainable Chemistry & Engineering
JF - ACS Sustainable Chemistry & Engineering
IS - 3
ER -