EGIII, a new endoglucanase from Trichoderma reesei

the characterization of both gene and enzyme

M. Saloheimo, P. Lehtovaara, M. Penttilä, T. T. Teeri, J. Ståhlberg, G. Johansson, G. Pettersson, M. Claeyssens, P. Tomme, J. K.C. Knowles

Research output: Contribution to journalArticleScientificpeer-review

260 Citations (Scopus)

Abstract

A novel endoglucanase from Trichoderma reesei, EGIII, has been purified and its catalytic properties have been studied. The gene for that enzyme (egl3) and cDNA have been cloned and sequenced. The deduced EGIII protein shows clear sequence homology to a Schizophyllum commune enzyme (M. Yaguchi, personal communication), but is very different from the three other T. reesei cellulases with known structure. Nevertheless, all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis. These homologous sequences reside at the N termini of EGIII and the cellobiohydrolase CBHII, but at the C termini of EGI and CBHI. Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites.

Original languageEnglish
Pages (from-to)11-21
Number of pages11
JournalGene
Volume63
Issue number1
DOIs
Publication statusPublished - 15 Mar 1988
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulases
Trichoderma
Cellulase
Sequence Homology
Schizophyllum
Cellulose 1,4-beta-Cellobiosidase
Fungal Structures
Enzymes
Proteolysis
Genes
Catalytic Domain
Complementary DNA
Communication
Proteins

Keywords

  • active site
  • cellulase
  • gene sequence
  • module shuffling
  • phage 1 vector
  • Recombinant DNA
  • Trichoderma

Cite this

Saloheimo, M. ; Lehtovaara, P. ; Penttilä, M. ; Teeri, T. T. ; Ståhlberg, J. ; Johansson, G. ; Pettersson, G. ; Claeyssens, M. ; Tomme, P. ; Knowles, J. K.C. / EGIII, a new endoglucanase from Trichoderma reesei : the characterization of both gene and enzyme. In: Gene. 1988 ; Vol. 63, No. 1. pp. 11-21.
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title = "EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme",
abstract = "A novel endoglucanase from Trichoderma reesei, EGIII, has been purified and its catalytic properties have been studied. The gene for that enzyme (egl3) and cDNA have been cloned and sequenced. The deduced EGIII protein shows clear sequence homology to a Schizophyllum commune enzyme (M. Yaguchi, personal communication), but is very different from the three other T. reesei cellulases with known structure. Nevertheless, all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis. These homologous sequences reside at the N termini of EGIII and the cellobiohydrolase CBHII, but at the C termini of EGI and CBHI. Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites.",
keywords = "active site, cellulase, gene sequence, module shuffling, phage 1 vector, Recombinant DNA, Trichoderma",
author = "M. Saloheimo and P. Lehtovaara and M. Penttil{\"a} and Teeri, {T. T.} and J. St{\aa}hlberg and G. Johansson and G. Pettersson and M. Claeyssens and P. Tomme and Knowles, {J. K.C.}",
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Saloheimo, M, Lehtovaara, P, Penttilä, M, Teeri, TT, Ståhlberg, J, Johansson, G, Pettersson, G, Claeyssens, M, Tomme, P & Knowles, JKC 1988, 'EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme', Gene, vol. 63, no. 1, pp. 11-21. https://doi.org/10.1016/0378-1119(88)90541-0

EGIII, a new endoglucanase from Trichoderma reesei : the characterization of both gene and enzyme. / Saloheimo, M.; Lehtovaara, P.; Penttilä, M.; Teeri, T. T.; Ståhlberg, J.; Johansson, G.; Pettersson, G.; Claeyssens, M.; Tomme, P.; Knowles, J. K.C.

In: Gene, Vol. 63, No. 1, 15.03.1988, p. 11-21.

Research output: Contribution to journalArticleScientificpeer-review

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T2 - the characterization of both gene and enzyme

AU - Saloheimo, M.

AU - Lehtovaara, P.

AU - Penttilä, M.

AU - Teeri, T. T.

AU - Ståhlberg, J.

AU - Johansson, G.

AU - Pettersson, G.

AU - Claeyssens, M.

AU - Tomme, P.

AU - Knowles, J. K.C.

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N2 - A novel endoglucanase from Trichoderma reesei, EGIII, has been purified and its catalytic properties have been studied. The gene for that enzyme (egl3) and cDNA have been cloned and sequenced. The deduced EGIII protein shows clear sequence homology to a Schizophyllum commune enzyme (M. Yaguchi, personal communication), but is very different from the three other T. reesei cellulases with known structure. Nevertheless, all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis. These homologous sequences reside at the N termini of EGIII and the cellobiohydrolase CBHII, but at the C termini of EGI and CBHI. Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites.

AB - A novel endoglucanase from Trichoderma reesei, EGIII, has been purified and its catalytic properties have been studied. The gene for that enzyme (egl3) and cDNA have been cloned and sequenced. The deduced EGIII protein shows clear sequence homology to a Schizophyllum commune enzyme (M. Yaguchi, personal communication), but is very different from the three other T. reesei cellulases with known structure. Nevertheless, all the four T. reesei cellulases share two common, adjacent sequence domains, which apparently can be removed by proteolysis. These homologous sequences reside at the N termini of EGIII and the cellobiohydrolase CBHII, but at the C termini of EGI and CBHI. Comparison of the fungal cellulase structures has led to re-evaluation of hypotheses concerning the localization of the active sites.

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KW - cellulase

KW - gene sequence

KW - module shuffling

KW - phage 1 vector

KW - Recombinant DNA

KW - Trichoderma

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