Elastic and pH responsive hybrid interfaces created with engineered resilin and nanocellulose

Wenwen Fang, Arja Paananen, Marika Vitikainen, Salla Koskela, Ann Westerholm-Parvinen, Jussi Joensuu, Christopher Landowski, Merja Penttilä, Markus B. Linder, Päivi Laaksonen

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.

Original languageEnglish
Pages (from-to)1866-1873
Number of pages8
JournalBiomacromolecules
Volume18
Issue number6
DOIs
Publication statusPublished - 12 Jun 2017
MoE publication typeA1 Journal article-refereed

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Cellulose
Proteins
Fusion reactions
Hydrogel
Polypeptides
Hydrogels
Swelling
Stiffness
Peptides
resilin

Cite this

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title = "Elastic and pH responsive hybrid interfaces created with engineered resilin and nanocellulose",
abstract = "We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.",
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Elastic and pH responsive hybrid interfaces created with engineered resilin and nanocellulose. / Fang, Wenwen; Paananen, Arja; Vitikainen, Marika; Koskela, Salla; Westerholm-Parvinen, Ann; Joensuu, Jussi; Landowski, Christopher; Penttilä, Merja; Linder, Markus B.; Laaksonen, Päivi.

In: Biomacromolecules, Vol. 18, No. 6, 12.06.2017, p. 1866-1873.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Elastic and pH responsive hybrid interfaces created with engineered resilin and nanocellulose

AU - Fang, Wenwen

AU - Paananen, Arja

AU - Vitikainen, Marika

AU - Koskela, Salla

AU - Westerholm-Parvinen, Ann

AU - Joensuu, Jussi

AU - Landowski, Christopher

AU - Penttilä, Merja

AU - Linder, Markus B.

AU - Laaksonen, Päivi

PY - 2017/6/12

Y1 - 2017/6/12

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AB - We investigated how a genetically engineered resilin fusion protein modifies cellulose surfaces. We characterized the pH-responsive behavior of a resilin-like polypeptide (RLP) having terminal cellulose binding modules (CBM) and showed its binding to cellulose nanofibrils (CNF). Characterization of the resilin fusion protein at different pHs revealed substantial conformational changes of the protein, which were observed as swelling and contraction of the protein layer bound to the nanocellulose surface. In addition, we showed that employment of the modified resilin in cellulose hydrogel and nanopaper increased their modulus of stiffness through a cross-linking effect.

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DO - 10.1021/acs.biomac.7b00294

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JO - Biomacromolecules

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