Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite

Jani Pelto (Corresponding Author), Suvi Haimi, Esa Puukilainen, Philip O. Whitten, Geoffrey M. Spinks, Mehrdad Bahrami-Samani, Mikko Ritala, Tommi Vuorinen

Research output: Contribution to journalArticleScientificpeer-review

22 Citations (Scopus)

Abstract

Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA‐CNT) was studied in situ by electrochemical atomic force microscopy (EC‐AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT‐cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST‐1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical‐QCM (EC‐QCM) analysis of a 210‐nm thick film reveals that the material is very soft G′∼100 kPa and swells upon reduction. EC‐AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20–25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA‐CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide.
Original languageEnglish
Pages (from-to)1056-1067
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume93
Issue number3
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyaluronic acid
Carbon Nanotubes
Polypyrroles
Hyaluronic Acid
Biocompatibility
Carbon nanotubes
Composite materials
Titanium
Stem cells
Atomic force microscopy
Gene Conversion
Quartz
Spectroscopic analysis
Quartz crystal microbalances
Microelectrodes
Cell proliferation
Thick films
Gold
Electrolytes
Cyclic voltammetry

Keywords

  • polypyrrole composite
  • electroactive polymer
  • atomic force microscopy
  • quartz crystal microbalance
  • biocompatibility

Cite this

Pelto, Jani ; Haimi, Suvi ; Puukilainen, Esa ; Whitten, Philip O. ; Spinks, Geoffrey M. ; Bahrami-Samani, Mehrdad ; Ritala, Mikko ; Vuorinen, Tommi. / Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 93, No. 3. pp. 1056-1067.
@article{d6d4717872c74316b49cc0fa21daeda4,
title = "Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite",
abstract = "Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA‐CNT) was studied in situ by electrochemical atomic force microscopy (EC‐AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT‐cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST‐1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20{\%} of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical‐QCM (EC‐QCM) analysis of a 210‐nm thick film reveals that the material is very soft G′∼100 kPa and swells upon reduction. EC‐AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20–25{\%} thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA‐CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide.",
keywords = "polypyrrole composite, electroactive polymer, atomic force microscopy, quartz crystal microbalance, biocompatibility",
author = "Jani Pelto and Suvi Haimi and Esa Puukilainen and Whitten, {Philip O.} and Spinks, {Geoffrey M.} and Mehrdad Bahrami-Samani and Mikko Ritala and Tommi Vuorinen",
year = "2010",
doi = "10.1002/jbm.a.32603",
language = "English",
volume = "93",
pages = "1056--1067",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "Wiley",
number = "3",

}

Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite. / Pelto, Jani (Corresponding Author); Haimi, Suvi; Puukilainen, Esa; Whitten, Philip O.; Spinks, Geoffrey M.; Bahrami-Samani, Mehrdad; Ritala, Mikko; Vuorinen, Tommi.

In: Journal of Biomedical Materials Research - Part A, Vol. 93, No. 3, 2010, p. 1056-1067.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Electroactivity and biocompatibility of polypyrrole-hyaluronic acid multi-walled carbon nanotube composite

AU - Pelto, Jani

AU - Haimi, Suvi

AU - Puukilainen, Esa

AU - Whitten, Philip O.

AU - Spinks, Geoffrey M.

AU - Bahrami-Samani, Mehrdad

AU - Ritala, Mikko

AU - Vuorinen, Tommi

PY - 2010

Y1 - 2010

N2 - Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA‐CNT) was studied in situ by electrochemical atomic force microscopy (EC‐AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT‐cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST‐1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical‐QCM (EC‐QCM) analysis of a 210‐nm thick film reveals that the material is very soft G′∼100 kPa and swells upon reduction. EC‐AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20–25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA‐CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide.

AB - Electroactivity of polypyrrole hyaluronic acid, electropolymerized in the presence of oxidized carbon nanotubes (PPyHA‐CNT) was studied in situ by electrochemical atomic force microscopy (EC‐AFM) in physiological electrolyte solution. In situ Raman spectroscopic and quartz crystal microbalance (QCM) studies were conducted on layers of the polymer grown on AT‐cut 5 MHz quartz crystals. Human adipose stem cell (ASC) attachment and viability were studied by Live/Dead staining, and the proliferation was evaluated by WST‐1 Cell proliferation assay for polypyrrole samples electropolymerized on titanium. According to cyclic voltammetry, the measured specific capacitance of the material on gold is roughly 20% of the reference polypyrrole dodecylbenzene sulfonate (PPyDBS). Electrochemical‐QCM (EC‐QCM) analysis of a 210‐nm thick film reveals that the material is very soft G′∼100 kPa and swells upon reduction. EC‐AFM of samples polymerized on microelectrodes show that there are areas of varying electroactivity, especially for samples without a hydrophopic backing PPyDBS layer. AFM line scans show typically 20–25% thickness change during electrochemical reduction. Raman spectroscopic analysis suggests that the material supports noticeable polaron conduction. Biocompatibility study of the PPyHA‐CNT on titanium with adipose stem cells showed equal or better cell attachment, viability, and proliferation compared with the reference polylactide.

KW - polypyrrole composite

KW - electroactive polymer

KW - atomic force microscopy

KW - quartz crystal microbalance

KW - biocompatibility

U2 - 10.1002/jbm.a.32603

DO - 10.1002/jbm.a.32603

M3 - Article

VL - 93

SP - 1056

EP - 1067

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 3

ER -