Electrochemical properties of honeycomb-like structured HFBI self-organized membranes on HOPG electrodes

R. Yamasaki, Y. Takatsuji, M. Lienemann, H. Asakawa, T. Fukuma, M. Linder, T. Haruyama

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

HFBI (derived from Trichoderma sp.) is a unique structural protein, which forms a self-organized monolayer at both air/water interface and water/solid interfaces in accurate two-dimensional ordered structures. We have taken advantage of the unique functionality of HFBI as a molecular carrier for preparation of ordered molecular phase on solid substrate surfaces. The HFBI molecular carrier can easily form ordered structures; however, the dense molecular layers form an electrochemical barrier between the electrode and solution phase. In this study, the electrochemical properties of HFBI self-organized membrane-covered electrodes were investigated. Wild-type HFBI has balanced positive and negative charges on its surface. Highly oriented pyrolytic graphite (HOPG) electrodes coated with HFBI molecules were investigated electrochemically. To improve the electrochemical properties of this HFBI-coated electrode, the two types of HFBI variants, with oppositely charged surfaces, were prepared genetically. All three types of HFBI-coated HOPG electrode perform electron transfer between the electrode and solution phase through the dense HFBI molecular layer. This is because the HFBI self-organized membrane has a honeycomb-like structure, with penetrating holes. In the cases of HFBI variants, the oppositely charged HFBI membrane phases shown opposite electrochemical behaviors in electrochemical impedance spectroscopy. HFBI is a molecule with a unique structure, and can easily form honeycomb-like structures on solid material surfaces such as electrodes. The molecular membrane phase can be used for electrochemical molecular interfaces.
Original languageEnglish
Pages (from-to)803-808
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Graphite electrodes
Graphite
pyrolytic graphite
Electrochemical properties
Electrodes
membranes
Membranes
electrodes
honeycomb structures
Molecules
Electrochemical impedance spectroscopy
Water
Monolayers
1-(heptafluorobutyryl)imidazole
Proteins
water
molecules
electron transfer
Electrons
Substrates

Keywords

  • self-assembled membrane
  • HFBI
  • electrochemical
  • modified electrode
  • impedance
  • engineered protein

Cite this

Yamasaki, R. ; Takatsuji, Y. ; Lienemann, M. ; Asakawa, H. ; Fukuma, T. ; Linder, M. ; Haruyama, T. / Electrochemical properties of honeycomb-like structured HFBI self-organized membranes on HOPG electrodes. In: Colloids and Surfaces B: Biointerfaces. 2014 ; Vol. 123. pp. 803-808.
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Electrochemical properties of honeycomb-like structured HFBI self-organized membranes on HOPG electrodes. / Yamasaki, R.; Takatsuji, Y.; Lienemann, M.; Asakawa, H.; Fukuma, T.; Linder, M.; Haruyama, T.

In: Colloids and Surfaces B: Biointerfaces, Vol. 123, 2014, p. 803-808.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Electrochemical properties of honeycomb-like structured HFBI self-organized membranes on HOPG electrodes

AU - Yamasaki, R.

AU - Takatsuji, Y.

AU - Lienemann, M.

AU - Asakawa, H.

AU - Fukuma, T.

AU - Linder, M.

AU - Haruyama, T.

PY - 2014

Y1 - 2014

N2 - HFBI (derived from Trichoderma sp.) is a unique structural protein, which forms a self-organized monolayer at both air/water interface and water/solid interfaces in accurate two-dimensional ordered structures. We have taken advantage of the unique functionality of HFBI as a molecular carrier for preparation of ordered molecular phase on solid substrate surfaces. The HFBI molecular carrier can easily form ordered structures; however, the dense molecular layers form an electrochemical barrier between the electrode and solution phase. In this study, the electrochemical properties of HFBI self-organized membrane-covered electrodes were investigated. Wild-type HFBI has balanced positive and negative charges on its surface. Highly oriented pyrolytic graphite (HOPG) electrodes coated with HFBI molecules were investigated electrochemically. To improve the electrochemical properties of this HFBI-coated electrode, the two types of HFBI variants, with oppositely charged surfaces, were prepared genetically. All three types of HFBI-coated HOPG electrode perform electron transfer between the electrode and solution phase through the dense HFBI molecular layer. This is because the HFBI self-organized membrane has a honeycomb-like structure, with penetrating holes. In the cases of HFBI variants, the oppositely charged HFBI membrane phases shown opposite electrochemical behaviors in electrochemical impedance spectroscopy. HFBI is a molecule with a unique structure, and can easily form honeycomb-like structures on solid material surfaces such as electrodes. The molecular membrane phase can be used for electrochemical molecular interfaces.

AB - HFBI (derived from Trichoderma sp.) is a unique structural protein, which forms a self-organized monolayer at both air/water interface and water/solid interfaces in accurate two-dimensional ordered structures. We have taken advantage of the unique functionality of HFBI as a molecular carrier for preparation of ordered molecular phase on solid substrate surfaces. The HFBI molecular carrier can easily form ordered structures; however, the dense molecular layers form an electrochemical barrier between the electrode and solution phase. In this study, the electrochemical properties of HFBI self-organized membrane-covered electrodes were investigated. Wild-type HFBI has balanced positive and negative charges on its surface. Highly oriented pyrolytic graphite (HOPG) electrodes coated with HFBI molecules were investigated electrochemically. To improve the electrochemical properties of this HFBI-coated electrode, the two types of HFBI variants, with oppositely charged surfaces, were prepared genetically. All three types of HFBI-coated HOPG electrode perform electron transfer between the electrode and solution phase through the dense HFBI molecular layer. This is because the HFBI self-organized membrane has a honeycomb-like structure, with penetrating holes. In the cases of HFBI variants, the oppositely charged HFBI membrane phases shown opposite electrochemical behaviors in electrochemical impedance spectroscopy. HFBI is a molecule with a unique structure, and can easily form honeycomb-like structures on solid material surfaces such as electrodes. The molecular membrane phase can be used for electrochemical molecular interfaces.

KW - self-assembled membrane

KW - HFBI

KW - electrochemical

KW - modified electrode

KW - impedance

KW - engineered protein

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EP - 808

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -