TY - JOUR
T1 - Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
AU - Havukainen, Sami
AU - Pujol-giménez, Jonai
AU - Valkonen, Mari
AU - Westerholm-parvinen, Ann
AU - Hediger, Matthias A.
AU - Landowski, Christopher P.
N1 - Funding Information:
This work was supported by Academy of Finland (funding decision No. 298392). Prof. Doris Rentsch of University of Bern is acknowledged for the use of her frog facility. Merja Helanterä is acknowledged for skillful technical assistance and Kari Koivuranta for providing yeast expression plasmids for STP1, STR1 and STR3, as well as GeneArt plasmids which were used for constructing CDT-1, CDT-2, Trire2_50618, Trire2_56684 (QM6a version), Trire2_72383 (QM6a version) and Trire2_79202 (QM6a version) expression plasmids. Prof. Matthias Hediger furthermore acknowledges the support of the electrophysiological functional studies of the transporters by the Swiss National Science Foundation Grant Sinergia #CRSII5 180326.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their production. Although this species has gained considerable interest in the scientific literature, the sugar transportome of T. reesei remains poorly characterized. Better understanding of the proteins involved in the transport of different sugars could be utilized for engineering better enzyme production strains. In this study we aimed to shed light on this matter by characterizing multiple T. reesei transporters capable of transporting various types of sugars. We used phylogenetics to select transporters for expression in Xenopus laevis oocytes to screen for transport activities. Of the 18 tested transporters, 8 were found to be functional in oocytes. 10 transporters in total were investigated in oocytes and in yeast, and for 3 of them no transport function had been described in literature. This comprehensive analysis provides a large body of new knowledge about T. reesei sugar transporters, and further establishes X. laevis oocytes as a valuable tool for studying fungal sugar transporters.
AB - Trichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their production. Although this species has gained considerable interest in the scientific literature, the sugar transportome of T. reesei remains poorly characterized. Better understanding of the proteins involved in the transport of different sugars could be utilized for engineering better enzyme production strains. In this study we aimed to shed light on this matter by characterizing multiple T. reesei transporters capable of transporting various types of sugars. We used phylogenetics to select transporters for expression in Xenopus laevis oocytes to screen for transport activities. Of the 18 tested transporters, 8 were found to be functional in oocytes. 10 transporters in total were investigated in oocytes and in yeast, and for 3 of them no transport function had been described in literature. This comprehensive analysis provides a large body of new knowledge about T. reesei sugar transporters, and further establishes X. laevis oocytes as a valuable tool for studying fungal sugar transporters.
UR - http://www.scopus.com/inward/record.url?scp=85110871414&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-93552-7
DO - 10.1038/s41598-021-93552-7
M3 - Article
C2 - 34282161
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 14678
ER -