Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

Kajsa Kajander, Eveliina Myllyluoma, Sinikka Kyrönpalo, Martin Rasmussen, Pentti Sipponen, Ismo Mattila, Tuulikki Seppänen-Laakso, Heikki Vapaatalo, Matej Oresic, Riitta Korpela (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.

METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.

RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).

CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.
Original languageEnglish
Pages (from-to)6068-6074
Number of pages7
JournalWorld Journal of Gastroenterology
Volume15
Issue number48
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Irritable Bowel Syndrome
Lipids
Metabolome
Ceramides
Healthy Volunteers
Lysophospholipids
Ascending Colon
Glycosphingolipids
Lysophosphatidylcholines
Metabolomics
Diglycerides
Esters
Mucous Membrane
Triglycerides
Up-Regulation
Biopsy

Keywords

  • Functional gastrointestinal diseases
  • irritable bowel syndrome
  • histopathology

Cite this

Kajander, K., Myllyluoma, E., Kyrönpalo, S., Rasmussen, M., Sipponen, P., Mattila, I., ... Korpela, R. (2009). Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome. World Journal of Gastroenterology, 15(48), 6068-6074. https://doi.org/10.3748/wjg.15.6068
Kajander, Kajsa ; Myllyluoma, Eveliina ; Kyrönpalo, Sinikka ; Rasmussen, Martin ; Sipponen, Pentti ; Mattila, Ismo ; Seppänen-Laakso, Tuulikki ; Vapaatalo, Heikki ; Oresic, Matej ; Korpela, Riitta. / Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome. In: World Journal of Gastroenterology. 2009 ; Vol. 15, No. 48. pp. 6068-6074.
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abstract = "AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.",
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Kajander, K, Myllyluoma, E, Kyrönpalo, S, Rasmussen, M, Sipponen, P, Mattila, I, Seppänen-Laakso, T, Vapaatalo, H, Oresic, M & Korpela, R 2009, 'Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome', World Journal of Gastroenterology, vol. 15, no. 48, pp. 6068-6074. https://doi.org/10.3748/wjg.15.6068

Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome. / Kajander, Kajsa; Myllyluoma, Eveliina; Kyrönpalo, Sinikka; Rasmussen, Martin; Sipponen, Pentti; Mattila, Ismo; Seppänen-Laakso, Tuulikki; Vapaatalo, Heikki; Oresic, Matej; Korpela, Riitta (Corresponding Author).

In: World Journal of Gastroenterology, Vol. 15, No. 48, 2009, p. 6068-6074.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

AU - Kajander, Kajsa

AU - Myllyluoma, Eveliina

AU - Kyrönpalo, Sinikka

AU - Rasmussen, Martin

AU - Sipponen, Pentti

AU - Mattila, Ismo

AU - Seppänen-Laakso, Tuulikki

AU - Vapaatalo, Heikki

AU - Oresic, Matej

AU - Korpela, Riitta

PY - 2009

Y1 - 2009

N2 - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

AB - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

KW - Functional gastrointestinal diseases

KW - irritable bowel syndrome

KW - histopathology

U2 - 10.3748/wjg.15.6068

DO - 10.3748/wjg.15.6068

M3 - Article

VL - 15

SP - 6068

EP - 6074

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 48

ER -

Kajander K, Myllyluoma E, Kyrönpalo S, Rasmussen M, Sipponen P, Mattila I et al. Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome. World Journal of Gastroenterology. 2009;15(48):6068-6074. https://doi.org/10.3748/wjg.15.6068