Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

Kajsa Kajander; Eveliina Myllyluoma; Sinikka Kyrönpalo; Martin Rasmussen; Pentti Sipponen; Ismo Mattila; Tuulikki Seppänen-Laakso; Heikki Vapaatalo; Matej Orešič; Riitta Korpela

doi:10.3748/wjg.15.6068

Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

Kajsa Kajander
, Eveliina Myllyluoma
, Sinikka Kyrönpalo
, Martin Rasmussen
, Pentti Sipponen
, Ismo Mattila
, Tuulikki Seppänen-Laakso
, Heikki Vapaatalo
, Matej Orešič
, Riitta Korpela^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

41 Citations (Scopus)

Abstract

AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls.

METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon.

RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03).

CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

Original language	English
Pages (from-to)	6068-6074
Journal	World Journal of Gastroenterology
Volume	15
Issue number	48
DOIs	https://doi.org/10.3748/wjg.15.6068
Publication status	Published - 2009
MoE publication type	A1 Journal article-refereed

Keywords

Functional gastrointestinal diseases
irritable bowel syndrome
histopathology

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10.3748/wjg.15.6068

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title = "Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome",

abstract = "AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon. RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03). CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.",

keywords = "Functional gastrointestinal diseases, irritable bowel syndrome, histopathology",

author = "Kajsa Kajander and Eveliina Myllyluoma and Sinikka Kyr{\"o}npalo and Martin Rasmussen and Pentti Sipponen and Ismo Mattila and Tuulikki Sepp{\"a}nen-Laakso and Heikki Vapaatalo and Matej Ore{\v s}i{\v c} and Riitta Korpela",

year = "2009",

doi = "10.3748/wjg.15.6068",

language = "English",

volume = "15",

pages = "6068--6074",

journal = "World Journal of Gastroenterology",

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publisher = "Baishideng Publishing Group Inc",

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TY - JOUR

T1 - Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

AU - Kajander, Kajsa

AU - Myllyluoma, Eveliina

AU - Kyrönpalo, Sinikka

AU - Rasmussen, Martin

AU - Sipponen, Pentti

AU - Mattila, Ismo

AU - Seppänen-Laakso, Tuulikki

AU - Vapaatalo, Heikki

AU - Orešič, Matej

AU - Korpela, Riitta

PY - 2009

Y1 - 2009

N2 - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon. RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03). CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

AB - AIM: To investigate the pathophysiology of irritable bowel syndrome (IBS) by comparing the global mucosal metabolic profiles of IBS patients with those of healthy controls. METHODS: Fifteen IBS patients fulfilling the Rome II criteria, and nine healthy volunteers were included in the study. A combined lipidomics (UPLC/MS) and metabolomics (GC × GC-TOF) approach was used to achieve global metabolic profiles of mucosal biopsies from the ascending colon. RESULTS: Overall, lipid levels were elevated in patients with IBS. The most significant upregulation was seen for pro-inflammatory lysophosphatidylcholines. Other lipid groups that were significantly upregulated in IBS patients were lipotoxic ceramides, glycosphingolipids, and di- and triacylglycerols. Among the metabolites, the cyclic ester 2(3H)-furanone was almost 14-fold upregulated in IBS patients compared to healthy subjects (P = 0.03). CONCLUSION: IBS mucosa is characterised by a distinct pro-inflammatory and lipotoxic metabolic profile. Especially, there was an increase in several lipid species such as lysophospholipids and ceramides.

KW - Functional gastrointestinal diseases

KW - irritable bowel syndrome

KW - histopathology

U2 - 10.3748/wjg.15.6068

DO - 10.3748/wjg.15.6068

M3 - Article

SN - 1007-9327

VL - 15

SP - 6068

EP - 6074

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

IS - 48

ER -

Elevated pro-inflammatory and lipotoxic mucosal lipids characterize irritable bowel syndrome

Abstract

Keywords

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