Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture

Philipp Meier, Hannu Hotti, Heiko Rischer (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Furanocoumarins, such as psoralen, xanthotoxin and bergapten, serve as protectants against phytopathogens and are used in pharmaceutical applications e.g. as DNA-crosslinking agents against non-melanoma skin cancers. Poison hemlock plants (Conium maculatum L.) are a known source of furanocoumarins and toxic alkaloids, but systematic research on callus and suspension cultures with the aim of eliciting secondary metabolites is lacking. Therefore callus cultures of poison hemlock were induced with 0.186 mg L?1 6-benzylaminopurine and 2 or 4 mg L?1 naphthalene acetic acid on McCown's Woody plant medium. A broad variety of elicitors (alginic acid, cellulase, chitosan, ethylene, methyl jasmonate, salicylic acid, copper(II) sulphate and silver nitrate) were tested with an established cell suspension culture for their capacity to trigger differential metabolite accumulation. Samples were extracted and analysed by gas chromatography-mass spectrometry. Elicitation with alginic acid, cellulase, chitosan, silver nitrate and copper(II) sulphate induced furanocoumarins. Plant hormones (ethylene, methyl jasmonate and salicylic acid) were not able to induce furanocoumarins. Extracts contained bergapten, columbianetin, isopimpinellin, marmesin, oroselone, psoralen and xanthotoxin but not piperidine alkaloids. The relative amount of furanocoumarins was generally higher in the medium than in the cells. The report describes the angular furanocoumarins oroselone and columbianetin together with the linear furanocoumarin marmesin, elicited for the first time in poison hemlock.
Original languageEnglish
Pages (from-to)443-453
JournalPlant Cell, Tissue and Organ Culture
Volume123
Issue number3
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Conium maculatum
psoralens
cell culture
bergapten
psoralen
methoxsalen
silver nitrate
copper sulfate
methyl jasmonate
alginates
chitosan
endo-1,4-beta-glucanase
salicylic acid
ethylene
piperidine alkaloids
callus culture
woody plants
naphthaleneacetic acid
plant pathogens
benzyladenine

Keywords

  • callus
  • cell culture
  • elicitation
  • furanocoumarins
  • poison hemlock (Conium maculatum L.)

Cite this

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title = "Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture",
abstract = "Furanocoumarins, such as psoralen, xanthotoxin and bergapten, serve as protectants against phytopathogens and are used in pharmaceutical applications e.g. as DNA-crosslinking agents against non-melanoma skin cancers. Poison hemlock plants (Conium maculatum L.) are a known source of furanocoumarins and toxic alkaloids, but systematic research on callus and suspension cultures with the aim of eliciting secondary metabolites is lacking. Therefore callus cultures of poison hemlock were induced with 0.186 mg L?1 6-benzylaminopurine and 2 or 4 mg L?1 naphthalene acetic acid on McCown's Woody plant medium. A broad variety of elicitors (alginic acid, cellulase, chitosan, ethylene, methyl jasmonate, salicylic acid, copper(II) sulphate and silver nitrate) were tested with an established cell suspension culture for their capacity to trigger differential metabolite accumulation. Samples were extracted and analysed by gas chromatography-mass spectrometry. Elicitation with alginic acid, cellulase, chitosan, silver nitrate and copper(II) sulphate induced furanocoumarins. Plant hormones (ethylene, methyl jasmonate and salicylic acid) were not able to induce furanocoumarins. Extracts contained bergapten, columbianetin, isopimpinellin, marmesin, oroselone, psoralen and xanthotoxin but not piperidine alkaloids. The relative amount of furanocoumarins was generally higher in the medium than in the cells. The report describes the angular furanocoumarins oroselone and columbianetin together with the linear furanocoumarin marmesin, elicited for the first time in poison hemlock.",
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author = "Philipp Meier and Hannu Hotti and Heiko Rischer",
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language = "English",
volume = "123",
pages = "443--453",
journal = "Plant Cell, Tissue and Organ Culture",
issn = "0167-6857",
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}

Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture. / Meier, Philipp; Hotti, Hannu; Rischer, Heiko (Corresponding Author).

In: Plant Cell, Tissue and Organ Culture, Vol. 123, No. 3, 2015, p. 443-453.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture

AU - Meier, Philipp

AU - Hotti, Hannu

AU - Rischer, Heiko

PY - 2015

Y1 - 2015

N2 - Furanocoumarins, such as psoralen, xanthotoxin and bergapten, serve as protectants against phytopathogens and are used in pharmaceutical applications e.g. as DNA-crosslinking agents against non-melanoma skin cancers. Poison hemlock plants (Conium maculatum L.) are a known source of furanocoumarins and toxic alkaloids, but systematic research on callus and suspension cultures with the aim of eliciting secondary metabolites is lacking. Therefore callus cultures of poison hemlock were induced with 0.186 mg L?1 6-benzylaminopurine and 2 or 4 mg L?1 naphthalene acetic acid on McCown's Woody plant medium. A broad variety of elicitors (alginic acid, cellulase, chitosan, ethylene, methyl jasmonate, salicylic acid, copper(II) sulphate and silver nitrate) were tested with an established cell suspension culture for their capacity to trigger differential metabolite accumulation. Samples were extracted and analysed by gas chromatography-mass spectrometry. Elicitation with alginic acid, cellulase, chitosan, silver nitrate and copper(II) sulphate induced furanocoumarins. Plant hormones (ethylene, methyl jasmonate and salicylic acid) were not able to induce furanocoumarins. Extracts contained bergapten, columbianetin, isopimpinellin, marmesin, oroselone, psoralen and xanthotoxin but not piperidine alkaloids. The relative amount of furanocoumarins was generally higher in the medium than in the cells. The report describes the angular furanocoumarins oroselone and columbianetin together with the linear furanocoumarin marmesin, elicited for the first time in poison hemlock.

AB - Furanocoumarins, such as psoralen, xanthotoxin and bergapten, serve as protectants against phytopathogens and are used in pharmaceutical applications e.g. as DNA-crosslinking agents against non-melanoma skin cancers. Poison hemlock plants (Conium maculatum L.) are a known source of furanocoumarins and toxic alkaloids, but systematic research on callus and suspension cultures with the aim of eliciting secondary metabolites is lacking. Therefore callus cultures of poison hemlock were induced with 0.186 mg L?1 6-benzylaminopurine and 2 or 4 mg L?1 naphthalene acetic acid on McCown's Woody plant medium. A broad variety of elicitors (alginic acid, cellulase, chitosan, ethylene, methyl jasmonate, salicylic acid, copper(II) sulphate and silver nitrate) were tested with an established cell suspension culture for their capacity to trigger differential metabolite accumulation. Samples were extracted and analysed by gas chromatography-mass spectrometry. Elicitation with alginic acid, cellulase, chitosan, silver nitrate and copper(II) sulphate induced furanocoumarins. Plant hormones (ethylene, methyl jasmonate and salicylic acid) were not able to induce furanocoumarins. Extracts contained bergapten, columbianetin, isopimpinellin, marmesin, oroselone, psoralen and xanthotoxin but not piperidine alkaloids. The relative amount of furanocoumarins was generally higher in the medium than in the cells. The report describes the angular furanocoumarins oroselone and columbianetin together with the linear furanocoumarin marmesin, elicited for the first time in poison hemlock.

KW - callus

KW - cell culture

KW - elicitation

KW - furanocoumarins

KW - poison hemlock (Conium maculatum L.)

U2 - 10.1007/s11240-015-0847-7

DO - 10.1007/s11240-015-0847-7

M3 - Article

VL - 123

SP - 443

EP - 453

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

SN - 0167-6857

IS - 3

ER -