Abstract
Furanocoumarins, such as psoralen, xanthotoxin and
bergapten, serve as protectants against phytopathogens
and are used in pharmaceutical applications e.g. as
DNA-crosslinking agents against non-melanoma skin
cancers. Poison hemlock plants (Conium maculatum L.) are
a known source of furanocoumarins and toxic alkaloids,
but systematic research on callus and suspension cultures
with the aim of eliciting secondary metabolites is
lacking. Therefore callus cultures of poison hemlock were
induced with 0.186 mg L?1 6-benzylaminopurine and 2 or 4
mg L?1 naphthalene acetic acid on McCown's Woody plant
medium. A broad variety of elicitors (alginic acid,
cellulase, chitosan, ethylene, methyl jasmonate,
salicylic acid, copper(II) sulphate and silver nitrate)
were tested with an established cell suspension culture
for their capacity to trigger differential metabolite
accumulation. Samples were extracted and analysed by gas
chromatography-mass spectrometry. Elicitation with
alginic acid, cellulase, chitosan, silver nitrate and
copper(II) sulphate induced furanocoumarins. Plant
hormones (ethylene, methyl jasmonate and salicylic acid)
were not able to induce furanocoumarins. Extracts
contained bergapten, columbianetin, isopimpinellin,
marmesin, oroselone, psoralen and xanthotoxin but not
piperidine alkaloids. The relative amount of
furanocoumarins was generally higher in the medium than
in the cells. The report describes the angular
furanocoumarins oroselone and columbianetin together with
the linear furanocoumarin marmesin, elicited for the
first time in poison hemlock.
Original language | English |
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Pages (from-to) | 443-453 |
Journal | Plant Cell, Tissue and Organ Culture |
Volume | 123 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2015 |
MoE publication type | A1 Journal article-refereed |
Keywords
- callus
- cell culture
- elicitation
- furanocoumarins
- poison hemlock (Conium maculatum L.)