ELISA kit for determination of egg white proteins: Interlaboratory study

K. Tomková, P. Cuhra, J. Rysová, P. Hanák, D. Gabrovská, Marja-Leena Laukkanen, Kristiina Takkinen

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

An interlaboratory study was conducted in 11 laboratories to validate an ELISA method developed for the quantitative determination of egg white proteins (EWPs) in foods. The ELISA kit used for this study is based on sheep polyclonal antibody. It does not produce any false-positive results or cross-reactivity in a broad food matrix range with zero EWP content. All participants obtained the Egg ELISA Kit-native with standard operational procedure and the list of samples, as well as the samples and a protocol for recording test results. The study included 10 food samples. Four samples of food matrix with zero EWP content showed EWP content lower than the first standard (EWP content 0.5 mg/kg). One sample of food matrix with zero EWP content revealed EWP content higher than standard 3 (1.5 mg EWP/kg). Five food samples containing EWP as an ingredient tested positive and one negative. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (1.4 mg EWP/kg) and LOD (0.43 mg EWP/kg), were calculated for the kit.
Original languageEnglish
Pages (from-to)1923-1929
Number of pages7
JournalJournal of AOAC International
Volume93
Issue number6
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Egg Proteins
egg albumen
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
egg
protein
protein content
proteins
Food
food matrix
food
sampling
matrix
foods
Statistical tests
Analysis of variance (ANOVA)
polyclonal antibodies
cross reaction
reproducibility
repeatability

Cite this

Tomková, K., Cuhra, P., Rysová, J., Hanák, P., Gabrovská, D., Laukkanen, M-L., & Takkinen, K. (2010). ELISA kit for determination of egg white proteins: Interlaboratory study. Journal of AOAC International, 93(6), 1923-1929.
Tomková, K. ; Cuhra, P. ; Rysová, J. ; Hanák, P. ; Gabrovská, D. ; Laukkanen, Marja-Leena ; Takkinen, Kristiina. / ELISA kit for determination of egg white proteins : Interlaboratory study. In: Journal of AOAC International. 2010 ; Vol. 93, No. 6. pp. 1923-1929.
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abstract = "An interlaboratory study was conducted in 11 laboratories to validate an ELISA method developed for the quantitative determination of egg white proteins (EWPs) in foods. The ELISA kit used for this study is based on sheep polyclonal antibody. It does not produce any false-positive results or cross-reactivity in a broad food matrix range with zero EWP content. All participants obtained the Egg ELISA Kit-native with standard operational procedure and the list of samples, as well as the samples and a protocol for recording test results. The study included 10 food samples. Four samples of food matrix with zero EWP content showed EWP content lower than the first standard (EWP content 0.5 mg/kg). One sample of food matrix with zero EWP content revealed EWP content higher than standard 3 (1.5 mg EWP/kg). Five food samples containing EWP as an ingredient tested positive and one negative. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (1.4 mg EWP/kg) and LOD (0.43 mg EWP/kg), were calculated for the kit.",
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Tomková, K, Cuhra, P, Rysová, J, Hanák, P, Gabrovská, D, Laukkanen, M-L & Takkinen, K 2010, 'ELISA kit for determination of egg white proteins: Interlaboratory study', Journal of AOAC International, vol. 93, no. 6, pp. 1923-1929.

ELISA kit for determination of egg white proteins : Interlaboratory study. / Tomková, K.; Cuhra, P.; Rysová, J.; Hanák, P.; Gabrovská, D.; Laukkanen, Marja-Leena; Takkinen, Kristiina.

In: Journal of AOAC International, Vol. 93, No. 6, 2010, p. 1923-1929.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - ELISA kit for determination of egg white proteins

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AU - Tomková, K.

AU - Cuhra, P.

AU - Rysová, J.

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AU - Gabrovská, D.

AU - Laukkanen, Marja-Leena

AU - Takkinen, Kristiina

PY - 2010

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AB - An interlaboratory study was conducted in 11 laboratories to validate an ELISA method developed for the quantitative determination of egg white proteins (EWPs) in foods. The ELISA kit used for this study is based on sheep polyclonal antibody. It does not produce any false-positive results or cross-reactivity in a broad food matrix range with zero EWP content. All participants obtained the Egg ELISA Kit-native with standard operational procedure and the list of samples, as well as the samples and a protocol for recording test results. The study included 10 food samples. Four samples of food matrix with zero EWP content showed EWP content lower than the first standard (EWP content 0.5 mg/kg). One sample of food matrix with zero EWP content revealed EWP content higher than standard 3 (1.5 mg EWP/kg). Five food samples containing EWP as an ingredient tested positive and one negative. The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as LOQ (1.4 mg EWP/kg) and LOD (0.43 mg EWP/kg), were calculated for the kit.

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Tomková K, Cuhra P, Rysová J, Hanák P, Gabrovská D, Laukkanen M-L et al. ELISA kit for determination of egg white proteins: Interlaboratory study. Journal of AOAC International. 2010;93(6):1923-1929.