ELISA kit for mustard protein determination: Interlaboratory study

P. Cuhra (Corresponding Author), D. Gabrovská, J. Rysová, P. Hanák, F. Stumr, Marja-Leena Laukkanen, Kristiina Takkinen

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

An interlaboratory study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative determination of mustard protein in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false-positive results or cross-reactivity with in-house validation for a broad range of food matrixes with no detectable mustard protein. All participants obtained the Mustard ELISA kit with standard operational procedures, a list of samples, samples, and a protocol for recording test results. The study included 15 food samples and two spiked samples. Seven food matrix samples of zero mustard content and four samples with mustard declared as an ingredient showed mustard protein content lower than that of the first standard (0.42 mg/kg). Four samples with mustard declared as an ingredient revealed mustard protein content above 12.5 mg/kg (the highest standard). The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as an LOQ (1.8 mg mustard proteins/kg) and LOD (0.5 mg mustard proteins/kg), for the kit were calculated.
Original languageEnglish
Pages (from-to)605-610
Number of pages6
JournalJournal of AOAC International
Volume94
Issue number2
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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Mustard Plant
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
protein
Proteins
food
proteins
food matrix
sampling
Food
ingredients
protein content
matrix
Statistical tests
Analysis of variance (ANOVA)
variance analysis
antibody
polyclonal antibodies
cross reaction
reproducibility

Cite this

Cuhra, P., Gabrovská, D., Rysová, J., Hanák, P., Stumr, F., Laukkanen, M-L., & Takkinen, K. (2011). ELISA kit for mustard protein determination: Interlaboratory study. Journal of AOAC International, 94(2), 605-610.
Cuhra, P. ; Gabrovská, D. ; Rysová, J. ; Hanák, P. ; Stumr, F. ; Laukkanen, Marja-Leena ; Takkinen, Kristiina. / ELISA kit for mustard protein determination : Interlaboratory study. In: Journal of AOAC International. 2011 ; Vol. 94, No. 2. pp. 605-610.
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Cuhra, P, Gabrovská, D, Rysová, J, Hanák, P, Stumr, F, Laukkanen, M-L & Takkinen, K 2011, 'ELISA kit for mustard protein determination: Interlaboratory study', Journal of AOAC International, vol. 94, no. 2, pp. 605-610.

ELISA kit for mustard protein determination : Interlaboratory study. / Cuhra, P. (Corresponding Author); Gabrovská, D.; Rysová, J.; Hanák, P.; Stumr, F.; Laukkanen, Marja-Leena; Takkinen, Kristiina.

In: Journal of AOAC International, Vol. 94, No. 2, 2011, p. 605-610.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Cuhra, P.

AU - Gabrovská, D.

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AU - Laukkanen, Marja-Leena

AU - Takkinen, Kristiina

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Cuhra P, Gabrovská D, Rysová J, Hanák P, Stumr F, Laukkanen M-L et al. ELISA kit for mustard protein determination: Interlaboratory study. Journal of AOAC International. 2011;94(2):605-610.