The applicability of a polyelectrolyte, Elvacite 2669, as a pseudostationary phase in capillary electrokinetic chromatography for the separation of some neutral hydrophobic compounds was investigated. In addition, its behavior in buffer solutions for capillary electrophoretic separations was studied by dynamic light scattering. With the polymer concentration held constant and the methanol-water ratio varied, study was made of the viscosity, the migration times of some neutral hydrophobic compounds, and the light scattering properties of the buffers. The effect of varying the polymer concentration on the separation of the compounds was also investigated. All studies were made under basic conditions. Data on the aggregation behavior, including the size and aggregation number of the polyelectrolyte under different conditions, are given and the capillary electrophoretic results are interpreted in terms of the dynamic light scattering data. In addition, the interaction of Elvacite 2669 with the fused silica wall was investigated, and the results showed that polymer adsorption onto the capillary wall occurred at polymer concentrations at and above 0.5 wt%. Both the capillary electrophoretic and dynamic light scattering results showed the behavior of Elvacite 2669 to be highly dependent on the methanol-water ratio in the buffer.
|Number of pages||9|
|Journal||Journal of Microcolumn Separations|
|Publication status||Published - 1998|
|MoE publication type||A1 Journal article-refereed|
- electrokinetic capillary chromatography
- micellar electrokinetic capillary chromatography
- light scattering