The enantioselective esterification of racemic ibuprofen with n-propanol by immobilized Mucor miehel lipase in supercritical carbon dioxide was studied. The enantiomeric excess of the product (eep) was 70 % at 15...20 % conversion. The enantioselectivity was faintly affected by temperature and the concentration of ibuprofen and lipase. The optimum temperature was 45 °C. The initial reaction rate increased with pressure, but enantioselectivity was not affected by pressure changes. The reaction rates in supercritical carbon dioxide at optimized conditions and in n-hexane were similar.
Rantakylä, M., & Aaltonen, O. (1994). Enantioselective esterification of ibuprofen in supercritical carbon dioxide by immobilized lipase. Biotechnology Letters, 16(8), 825-830. https://doi.org/10.1007/BF00133961