Energy, control and DNA structure in the living cell

J. E. Wijker, P. R. Jensen, J. L. Snoep, A. Vaz Gomes, M. Guiral, A. P.M. Jongsma, A. de Waal, S. Hoving, S. van Dooren, C. C. van der Weijden, M. van Workum, W. C. van Heeswijk, O. Molenaar, P. Wielinga, P. Richard, J. Diderich, B. M. Bakker, B. Teusink, M. Hemker, J. M. RohwerA. A. van der Gugten, B. N. Kholodenko, H. V. Westerhoff

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)


Maintenance (let alone growth) of the highly ordered living cell is only possible through the continuous input of free energy. Coupling of energetically downhill processes (such as catabolic reactions) to uphill processes is essential to provide this free energy and is catalyzed by enzymes either directly or via "storage" in an intermediate high energy form, i.e., high ATP/ADP ratio or H+ ion gradient. Although maintenance of a sufficiently high ATP/ADP ratio is essential to overcome the thermodynamic burden of uphill processes, it is not clear to what degree enzymes that control this ratio also control cell physiology. Indeed, in the living cell homeostatic control mechanisms might exist for the free-energy transduction pathways so as to prevent perturbation of cellular function when the Gibbs energy supply is compromised. This presentation addresse the extent to which the intracellular ATP level is involved in the control of cell physiology, how the elaborate control of cell function may be analyzed theoretically and quantitatively, and if this can be utilized selectively to affect certain cell types.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalBiophysical Chemistry
Issue number1-2
Publication statusPublished - 1 Jan 1995
MoE publication typeNot Eligible


  • Cascade regulation
  • DNA supercoiling
  • Glutamine synthetase
  • Magainins
  • Metabolic control
  • Multidrug resistance


Dive into the research topics of 'Energy, control and DNA structure in the living cell'. Together they form a unique fingerprint.

Cite this