Engineering a filamentous fungus for l-rhamnose extraction

Joosu Kuivanen, Peter Richard

    Research output: Contribution to journalArticleScientificpeer-review

    8 Citations (Scopus)


    l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.
    Original languageEnglish
    JournalAMB Express
    Issue number27
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed


    • l-rhamnose
    • Rhamogalacturonan
    • pectin
    • citrus peel
    • aspergillus niger
    • metabolic engineering
    • consolidated bioprocess


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