Abstract
l-Rhamnose is a high value rare sugar that is used as
such or after chemical conversions. It is enriched in
several biomass fractions such as the pectic
polysaccharides rhamnogalacturonan I and II and in
naringin, hesperidin, rutin, quercitrin and ulvan. We
engineered the filamentous fungus Aspergillus niger to
not consume l-rhamnose, while it is still able to produce
the enzymes for the hydrolysis of l-rhamnose rich
biomass. As a result we present a strain that can be used
for the extraction of l-rhamnose in a consolidated
process. In the process the biomass is hydrolysed to the
monomeric sugars which are consumed by the fungus leaving
the l-rhamnose.
| Original language | English |
|---|---|
| Journal | AMB Express |
| Volume | 6 |
| Issue number | 27 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- l-rhamnose
- Rhamogalacturonan
- pectin
- citrus peel
- aspergillus niger
- metabolic engineering
- consolidated bioprocess
