Engineering a filamentous fungus for l-rhamnose extraction

Joosu Kuivanen, Peter Richard

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.
Original languageEnglish
JournalAMB Express
Volume6
Issue number27
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Rhamnose
Fungi
Biomass
Hesperidin
Rutin
Aspergillus niger
Polysaccharides
Hydrolysis
Enzymes

Keywords

  • l-rhamnose
  • Rhamogalacturonan
  • pectin
  • citrus peel
  • aspergillus niger
  • metabolic engineering
  • consolidated bioprocess

Cite this

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title = "Engineering a filamentous fungus for l-rhamnose extraction",
abstract = "l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.",
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Engineering a filamentous fungus for l-rhamnose extraction. / Kuivanen, Joosu; Richard, Peter.

In: AMB Express, Vol. 6, No. 27, 2016.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Engineering a filamentous fungus for l-rhamnose extraction

AU - Kuivanen, Joosu

AU - Richard, Peter

N1 - Project code: 100686

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N2 - l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.

AB - l-Rhamnose is a high value rare sugar that is used as such or after chemical conversions. It is enriched in several biomass fractions such as the pectic polysaccharides rhamnogalacturonan I and II and in naringin, hesperidin, rutin, quercitrin and ulvan. We engineered the filamentous fungus Aspergillus niger to not consume l-rhamnose, while it is still able to produce the enzymes for the hydrolysis of l-rhamnose rich biomass. As a result we present a strain that can be used for the extraction of l-rhamnose in a consolidated process. In the process the biomass is hydrolysed to the monomeric sugars which are consumed by the fungus leaving the l-rhamnose.

KW - l-rhamnose

KW - Rhamogalacturonan

KW - pectin

KW - citrus peel

KW - aspergillus niger

KW - metabolic engineering

KW - consolidated bioprocess

U2 - 10.1186/s13568-016-0198-8

DO - 10.1186/s13568-016-0198-8

M3 - Article

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JO - AMB Express

JF - AMB Express

SN - 2191-0855

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