Engineering Saccharomyces cerevisiae for production of glycolic acid

Research output: Contribution to conferenceConference PosterScientific

Abstract

Glycolic acid is the smallest alpha-hydroxy acid and is used in a wide range of chemical processes, in cosmetics and as a precursor for biopolymers. Currently glycolic acid is produced by chemical synthesis, for example, from chloroacetic acid. However, from a sustainable point of view a biotechnological production route would be more desirable. Glycolic acid production from lignocellulosic second generation biomass feedstocks would be sustainably and perhaps as well economically better solution.

Our approach was to use genetically engineered S. cerevisiae to develop a fermentation based method for glycolic acid production. Glycolic acid can be produced in the glyoxylate cycle where glyoxylate can be further converted to glycolic acid. S. cerevisiae has a gene coding for glyoxylate reductase but in order to make the conversion more efficient we overexpressed an NADPH specific glyoxylate reductase from Arabidobsis thaliana for converting glyoxylate to glycolic acid.

For efficient conversion of glyoxylate into glycolic acid and boosting up the glyoxylate cycle also further modifications were made. Two malate synthase genes were deleted to prevent glyoxylate to react to malate. Additionally steps leading to glyoxylate formation were boosted by overexpressing isocitrate lyase and aconitase. Also the influence of regulatory factors, effect of ethanol metabolism and acetyl-CoA and NADPH availability were tested. The resulting strains were cultivated in shake flasks in different media and the production of glycolic acid was demonstrated.
Original languageEnglish
Publication statusPublished - 2012
Event34th Symposium on Biotechnology for Fuels and Chemicals - New Orleans, United States
Duration: 30 Apr 20123 May 2012
Conference number: 34

Conference

Conference34th Symposium on Biotechnology for Fuels and Chemicals
CountryUnited States
CityNew Orleans
Period30/04/123/05/12

Fingerprint

glycolic acid
Saccharomyces cerevisiae
engineering
glyoxylate cycle
NADP (coenzyme)
malate synthase
isocitrate lyase
aconitate hydratase
acids
biopolymers
malates
cosmetics
feedstocks
genes
ethanol
fermentation

Cite this

Koivistoinen, O., Kuivanen, J., Penttilä, M., & Richard, P. (2012). Engineering Saccharomyces cerevisiae for production of glycolic acid. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.
Koivistoinen, Outi ; Kuivanen, Joosu ; Penttilä, Merja ; Richard, Peter. / Engineering Saccharomyces cerevisiae for production of glycolic acid. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.
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Koivistoinen, O, Kuivanen, J, Penttilä, M & Richard, P 2012, 'Engineering Saccharomyces cerevisiae for production of glycolic acid' 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States, 30/04/12 - 3/05/12, .

Engineering Saccharomyces cerevisiae for production of glycolic acid. / Koivistoinen, Outi; Kuivanen, Joosu; Penttilä, Merja; Richard, Peter.

2012. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Engineering Saccharomyces cerevisiae for production of glycolic acid

AU - Koivistoinen, Outi

AU - Kuivanen, Joosu

AU - Penttilä, Merja

AU - Richard, Peter

N1 - CA2: TK402 CA2: TK400

PY - 2012

Y1 - 2012

N2 - Glycolic acid is the smallest alpha-hydroxy acid and is used in a wide range of chemical processes, in cosmetics and as a precursor for biopolymers. Currently glycolic acid is produced by chemical synthesis, for example, from chloroacetic acid. However, from a sustainable point of view a biotechnological production route would be more desirable. Glycolic acid production from lignocellulosic second generation biomass feedstocks would be sustainably and perhaps as well economically better solution.Our approach was to use genetically engineered S. cerevisiae to develop a fermentation based method for glycolic acid production. Glycolic acid can be produced in the glyoxylate cycle where glyoxylate can be further converted to glycolic acid. S. cerevisiae has a gene coding for glyoxylate reductase but in order to make the conversion more efficient we overexpressed an NADPH specific glyoxylate reductase from Arabidobsis thaliana for converting glyoxylate to glycolic acid.For efficient conversion of glyoxylate into glycolic acid and boosting up the glyoxylate cycle also further modifications were made. Two malate synthase genes were deleted to prevent glyoxylate to react to malate. Additionally steps leading to glyoxylate formation were boosted by overexpressing isocitrate lyase and aconitase. Also the influence of regulatory factors, effect of ethanol metabolism and acetyl-CoA and NADPH availability were tested. The resulting strains were cultivated in shake flasks in different media and the production of glycolic acid was demonstrated.

AB - Glycolic acid is the smallest alpha-hydroxy acid and is used in a wide range of chemical processes, in cosmetics and as a precursor for biopolymers. Currently glycolic acid is produced by chemical synthesis, for example, from chloroacetic acid. However, from a sustainable point of view a biotechnological production route would be more desirable. Glycolic acid production from lignocellulosic second generation biomass feedstocks would be sustainably and perhaps as well economically better solution.Our approach was to use genetically engineered S. cerevisiae to develop a fermentation based method for glycolic acid production. Glycolic acid can be produced in the glyoxylate cycle where glyoxylate can be further converted to glycolic acid. S. cerevisiae has a gene coding for glyoxylate reductase but in order to make the conversion more efficient we overexpressed an NADPH specific glyoxylate reductase from Arabidobsis thaliana for converting glyoxylate to glycolic acid.For efficient conversion of glyoxylate into glycolic acid and boosting up the glyoxylate cycle also further modifications were made. Two malate synthase genes were deleted to prevent glyoxylate to react to malate. Additionally steps leading to glyoxylate formation were boosted by overexpressing isocitrate lyase and aconitase. Also the influence of regulatory factors, effect of ethanol metabolism and acetyl-CoA and NADPH availability were tested. The resulting strains were cultivated in shake flasks in different media and the production of glycolic acid was demonstrated.

M3 - Conference Poster

ER -

Koivistoinen O, Kuivanen J, Penttilä M, Richard P. Engineering Saccharomyces cerevisiae for production of glycolic acid. 2012. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.