Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei

Emilio Margolles-Clark, Gary E. Harman, Merja Penttilä

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.

Original languageEnglish
Pages (from-to)2152-2155
Number of pages4
JournalApplied and Environmental Microbiology
Volume62
Issue number6
Publication statusPublished - 1 Jan 1996
MoE publication typeA1 Journal article-refereed

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