Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei

Emilio Margolles-Clark, Gary E. Harman, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.

Original languageEnglish
Pages (from-to)2152-2155
Number of pages4
JournalApplied and Environmental Microbiology
Volume62
Issue number6
Publication statusPublished - 1 Jan 1996
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma reesei
Chitinases
Trichoderma
Trichoderma harzianum
sucrose
endo-1,4-beta-glucanase
glucose
mycoparasite
Cellulase
promoter regions
fold
mycoparasites
Sucrose
chitin
Glucose
crab
crabs
Chitin
fungus
shell

Cite this

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title = "Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei",
abstract = "Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.",
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Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei. / Margolles-Clark, Emilio; Harman, Gary E.; Penttilä, Merja.

In: Applied and Environmental Microbiology, Vol. 62, No. 6, 01.01.1996, p. 2152-2155.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei

AU - Margolles-Clark, Emilio

AU - Harman, Gary E.

AU - Penttilä, Merja

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AB - Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochitinase gene was expressed at a low basal level on glucose and sucrose. No specific induction by crab shell chitin or sophorose was observed.

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