Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 3956-3964 |
| Journal | Applied and Environmental Microbiology |
| Volume | 68 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2002 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Trichoderma reesei
- endoglucanase
- cellobiohydrolase
- cellulase
- stonewashing
- denim fabric
- denims
- clothes
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Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric. / Miettinen-Oinonen, Arja (Corresponding Author); Suominen, Pirkko.
In: Applied and Environmental Microbiology, Vol. 68, No. 8, 2002, p. 3956-3964.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric
AU - Miettinen-Oinonen, Arja
AU - Suominen, Pirkko
PY - 2002
Y1 - 2002
N2 - Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.
AB - Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.
KW - Trichoderma reesei
KW - endoglucanase
KW - cellobiohydrolase
KW - cellulase
KW - stonewashing
KW - denim fabric
KW - denims
KW - clothes
U2 - 10.1128/AEM.68.8.3956-3964.2002
DO - 10.1128/AEM.68.8.3956-3964.2002
M3 - Article
VL - 68
SP - 3956
EP - 3964
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 8
ER -