Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric

Arja Miettinen-Oinonen (Corresponding Author), Pirkko Suominen

Research output: Contribution to journalArticleScientificpeer-review

98 Citations (Scopus)

Abstract

Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.
Original languageEnglish
Pages (from-to)3956-3964
JournalApplied and Environmental Microbiology
Volume68
Issue number8
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

denim
Cellulose 1,4-beta-Cellobiosidase
Trichoderma reesei
Trichoderma
Cellulase
endo-1,4-beta-glucanase
cellulose 1,4-beta-cellobiosidase
fold
enzyme
filter
gene
host strains
fabric
effect
Enzymes
promoter regions
Genes
loci
endoglucanase 2

Keywords

  • Trichoderma reesei
  • endoglucanase
  • cellobiohydrolase
  • cellulase
  • stonewashing
  • denim fabric
  • denims
  • clothes

Cite this

@article{712a8bfb69ff4345816951cbfafcef6e,
title = "Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric",
abstract = "Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.",
keywords = "Trichoderma reesei, endoglucanase, cellobiohydrolase, cellulase, stonewashing, denim fabric, denims, clothes",
author = "Arja Miettinen-Oinonen and Pirkko Suominen",
year = "2002",
doi = "10.1128/AEM.68.8.3956-3964.2002",
language = "English",
volume = "68",
pages = "3956--3964",
journal = "Applied and Environmental Microbiology",
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publisher = "American Society for Microbiology",
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}

Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric. / Miettinen-Oinonen, Arja (Corresponding Author); Suominen, Pirkko.

In: Applied and Environmental Microbiology, Vol. 68, No. 8, 2002, p. 3956-3964.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enhanced production of Trichoderma reesei endoglucanases and use of the new cellulase preparations in producing the stonewashed effect on denim fabric

AU - Miettinen-Oinonen, Arja

AU - Suominen, Pirkko

PY - 2002

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N2 - Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.

AB - Trichoderma reesei strains were constructed for production of elevated amounts of endoglucanase II (EGII) with or without cellobiohydrolase I (CBHI). The endoglucanase activity produced by the EGII transformants correlated with the copy number of the egl2 expression cassette. One copy of the egl2 expression cassette in which the egl2 was under the cbh1 promoter increased production of endoglucanase activity 2.3-fold, and two copies increased production about 3-fold above that of the parent strain. When the enzyme with elevated EGII content was used, an improved stonewashing effect on denim fabric was achieved. A T. reesei strain producing high amounts of EGI and -II activities without CBHI and -II was constructed by replacing the cbh2 locus with the coding region of the egl2 gene in the EGI-overproducing CBHI-negative strain. Production of endoglucanase activity by the EG-transformant strain was increased fourfold above that of the host strain. The filter paper-degrading activity of the endoglucanase-overproducing strain was lowered to below detection, presumably because of the lack of cellobiohydrolases.

KW - Trichoderma reesei

KW - endoglucanase

KW - cellobiohydrolase

KW - cellulase

KW - stonewashing

KW - denim fabric

KW - denims

KW - clothes

U2 - 10.1128/AEM.68.8.3956-3964.2002

DO - 10.1128/AEM.68.8.3956-3964.2002

M3 - Article

VL - 68

SP - 3956

EP - 3964

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 8

ER -