Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

Suvi T. Häkkinen (Corresponding Author), E. Moyano, R. M. Cusidó, J. Palazón, M. T. Piñol, Kirsi-Marja Oksman-Caldentey

    Research output: Contribution to journalArticleScientificpeer-review

    72 Citations (Scopus)


    Hyoscyamine-6β-hydroxylase (H6H; EC catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus muticus. The transformation was performed using a binary vector system based on Agrobacterium rhizogenes. Production of scopolamine in hairy roots was clearly correlated with the 35S-h6h transcript expression. The engineered N. tabacum and H. muticus hairy roots were studied for the production of scopolamine and other tropane and nicotine alkaloids after feeding the cultures with exogenous hyoscyamine. N. tabacum hairy roots carrying the 35S-h6h transgene showed a more efficient uptake of hyoscyamine from the culture medium and a higher rate of bioconversion of hyoscyamine to scopolamine than those of H. muticus. In particular, the secretion of scopolamine in N. tabacum hairy roots was remarkably high, up to 85% of the total scopolamine being released to the culture medium. Exogenous hyoscyamine also caused changes in nicotine alkaloid accumulation in N. tabacum hairy roots.
    Original languageEnglish
    Pages (from-to)2611-2618
    Number of pages8
    JournalJournal of Experimental Botany
    Issue number420
    Publication statusPublished - Oct 2005
    MoE publication typeA1 Journal article-refereed


    • Hairy roots
    • Hyoscyamine-6beta-hydroxylase
    • Hyoscyamus
    • Nicotiana
    • Secretion
    • Tropane alkaloids


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