Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

Suvi T. Häkkinen (Corresponding Author), E. Moyano, R. M. Cusidó, J. Palazón, M. T. Piñol, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

60 Citations (Scopus)

Abstract

Hyoscyamine-6β-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus muticus. The transformation was performed using a binary vector system based on Agrobacterium rhizogenes. Production of scopolamine in hairy roots was clearly correlated with the 35S-h6h transcript expression. The engineered N. tabacum and H. muticus hairy roots were studied for the production of scopolamine and other tropane and nicotine alkaloids after feeding the cultures with exogenous hyoscyamine. N. tabacum hairy roots carrying the 35S-h6h transgene showed a more efficient uptake of hyoscyamine from the culture medium and a higher rate of bioconversion of hyoscyamine to scopolamine than those of H. muticus. In particular, the secretion of scopolamine in N. tabacum hairy roots was remarkably high, up to 85% of the total scopolamine being released to the culture medium. Exogenous hyoscyamine also caused changes in nicotine alkaloid accumulation in N. tabacum hairy roots.
Original languageEnglish
Pages (from-to)2611-2618
Number of pages8
JournalJournal of Experimental Botany
Volume56
Issue number420
DOIs
Publication statusPublished - Oct 2005
MoE publication typeA1 Journal article-refereed

Keywords

  • Hairy roots
  • Hyoscyamine-6beta-hydroxylase
  • Hyoscyamus
  • Nicotiana
  • Secretion
  • Tropane alkaloids

Fingerprint Dive into the research topics of 'Enhanced secretion of tropane alkaloids in <em>Nicotiana tabacum</em> hairy roots expressing heterologous hyoscyamine-6β-hydroxylase'. Together they form a unique fingerprint.

  • Cite this