Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2611-2618 |
| Number of pages | 8 |
| Journal | Journal of Experimental Botany |
| Volume | 56 |
| Issue number | 420 |
| DOIs | |
| Publication status | Published - Oct 2005 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Hairy roots
- Hyoscyamine-6beta-hydroxylase
- Hyoscyamus
- Nicotiana
- Secretion
- Tropane alkaloids
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Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase. / Häkkinen, Suvi T. (Corresponding Author); Moyano, E.; Cusidó, R. M.; Palazón, J.; Piñol, M. T.; Oksman-Caldentey, Kirsi-Marja.
In: Journal of Experimental Botany, Vol. 56, No. 420, 10.2005, p. 2611-2618.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase
AU - Häkkinen, Suvi T.
AU - Moyano, E.
AU - Cusidó, R. M.
AU - Palazón, J.
AU - Piñol, M. T.
AU - Oksman-Caldentey, Kirsi-Marja
PY - 2005/10
Y1 - 2005/10
N2 - Hyoscyamine-6β-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus muticus. The transformation was performed using a binary vector system based on Agrobacterium rhizogenes. Production of scopolamine in hairy roots was clearly correlated with the 35S-h6h transcript expression. The engineered N. tabacum and H. muticus hairy roots were studied for the production of scopolamine and other tropane and nicotine alkaloids after feeding the cultures with exogenous hyoscyamine. N. tabacum hairy roots carrying the 35S-h6h transgene showed a more efficient uptake of hyoscyamine from the culture medium and a higher rate of bioconversion of hyoscyamine to scopolamine than those of H. muticus. In particular, the secretion of scopolamine in N. tabacum hairy roots was remarkably high, up to 85% of the total scopolamine being released to the culture medium. Exogenous hyoscyamine also caused changes in nicotine alkaloid accumulation in N. tabacum hairy roots.
AB - Hyoscyamine-6β-hydroxylase (H6H; EC 1.14.11.11) catalyses oxidative reactions in the biosynthetic pathway leading from hyoscyamine to the more pharmaceutically valuable tropane alkaloid scopolamine. The h6h gene encoding H6H from Hyoscyamus niger was introduced, under the control of the CaMV 35S promoter, into non-hyoscyamine-producing Nicotiana tabacum and hyoscyamine-producing Hyoscyamus muticus. The transformation was performed using a binary vector system based on Agrobacterium rhizogenes. Production of scopolamine in hairy roots was clearly correlated with the 35S-h6h transcript expression. The engineered N. tabacum and H. muticus hairy roots were studied for the production of scopolamine and other tropane and nicotine alkaloids after feeding the cultures with exogenous hyoscyamine. N. tabacum hairy roots carrying the 35S-h6h transgene showed a more efficient uptake of hyoscyamine from the culture medium and a higher rate of bioconversion of hyoscyamine to scopolamine than those of H. muticus. In particular, the secretion of scopolamine in N. tabacum hairy roots was remarkably high, up to 85% of the total scopolamine being released to the culture medium. Exogenous hyoscyamine also caused changes in nicotine alkaloid accumulation in N. tabacum hairy roots.
KW - Hairy roots
KW - Hyoscyamine-6beta-hydroxylase
KW - Hyoscyamus
KW - Nicotiana
KW - Secretion
KW - Tropane alkaloids
U2 - 10.1093/jxb/eri253
DO - 10.1093/jxb/eri253
M3 - Article
C2 - 16105856
VL - 56
SP - 2611
EP - 2618
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 420
ER -