Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique

E. V. Khaydukov; V. A. Semchishen; V. N. Seminogov; V. I. Sokolov; Alexey P. Popov; A. V. Bykov; A. V. Nechaev; A. S. Akhmanov; V. Ya Panchenko; A. V. Zvyagin

doi:10.1088/1612-2011/11/9/095602

Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique

E. V. Khaydukov, V. A. Semchishen, V. N. Seminogov, V. I. Sokolov, Alexey P. Popov, A. V. Bykov, A. V. Nechaev, A. S. Akhmanov, V. Ya Panchenko, A. V. Zvyagin

Not published at VTT

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17 Citations (Scopus)

Abstract

The new generation of synthetic nanomaterials, upconversion nanoparticles (UCNPs), have the potential for high-contrast optical imaging of biological tissue by virtue of their unique luminescent properties which enable the autofluorescence and excitation signals to be completely suppressed and avoid biotissue absorption. The potential for deep tissue imaging, such as whole animal imaging, is demonstrated in this report on a comparative study of two epiluminescent imaging methods suitable for the localization of a UCNP-labelled pathology site buried in highly scattering biological tissue modelled by an optical tissue phantom. The lateral resolution exhibited in scanning imaging by an illumination-collection fibre-optic probe appeared to be almost 1.73 times better than that shown by the wide-field CCD commonly used in diffuse optical tomography systems. We attribute this improved lateral resolution to the enhanced angular selectivity of the illumination-collection regime and to the nonlinear dependence of the UCNP luminescence on the excitation intensity.

Original language	English
Article number	095602
Journal	Laser Physics Letters
Volume	11
Issue number	9
DOIs	https://doi.org/10.1088/1612-2011/11/9/095602
Publication status	Published - Sept 2014
MoE publication type	A1 Journal article-refereed

Keywords

Biophotonics
Diffuse optical tomography
Fibre-optic probe
Optical imaging
Tissue optics
Tissue phantom
Upconversion nanoparticles

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10.1088/1612-2011/11/9/095602

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Khaydukov, E. V., Semchishen, V. A., Seminogov, V. N., Sokolov, V. I., Popov, A. P., Bykov, A. V., Nechaev, A. V., Akhmanov, A. S., Panchenko, V. Y., & Zvyagin, A. V. (2014). Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique. Laser Physics Letters, 11(9), Article 095602. https://doi.org/10.1088/1612-2011/11/9/095602

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title = "Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique",

abstract = "The new generation of synthetic nanomaterials, upconversion nanoparticles (UCNPs), have the potential for high-contrast optical imaging of biological tissue by virtue of their unique luminescent properties which enable the autofluorescence and excitation signals to be completely suppressed and avoid biotissue absorption. The potential for deep tissue imaging, such as whole animal imaging, is demonstrated in this report on a comparative study of two epiluminescent imaging methods suitable for the localization of a UCNP-labelled pathology site buried in highly scattering biological tissue modelled by an optical tissue phantom. The lateral resolution exhibited in scanning imaging by an illumination-collection fibre-optic probe appeared to be almost 1.73 times better than that shown by the wide-field CCD commonly used in diffuse optical tomography systems. We attribute this improved lateral resolution to the enhanced angular selectivity of the illumination-collection regime and to the nonlinear dependence of the UCNP luminescence on the excitation intensity.",

keywords = "Biophotonics, Diffuse optical tomography, Fibre-optic probe, Optical imaging, Tissue optics, Tissue phantom, Upconversion nanoparticles",

author = "Khaydukov, {E. V.} and Semchishen, {V. A.} and Seminogov, {V. N.} and Sokolov, {V. I.} and Popov, {Alexey P.} and Bykov, {A. V.} and Nechaev, {A. V.} and Akhmanov, {A. S.} and Panchenko, {V. Ya} and Zvyagin, {A. V.}",

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Khaydukov, EV, Semchishen, VA, Seminogov, VN, Sokolov, VI, Popov, AP, Bykov, AV, Nechaev, AV, Akhmanov, AS, Panchenko, VY & Zvyagin, AV 2014, 'Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique', Laser Physics Letters, vol. 11, no. 9, 095602. https://doi.org/10.1088/1612-2011/11/9/095602

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AU - Sokolov, V. I.

AU - Popov, Alexey P.

AU - Bykov, A. V.

AU - Nechaev, A. V.

AU - Akhmanov, A. S.

AU - Panchenko, V. Ya

AU - Zvyagin, A. V.

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AB - The new generation of synthetic nanomaterials, upconversion nanoparticles (UCNPs), have the potential for high-contrast optical imaging of biological tissue by virtue of their unique luminescent properties which enable the autofluorescence and excitation signals to be completely suppressed and avoid biotissue absorption. The potential for deep tissue imaging, such as whole animal imaging, is demonstrated in this report on a comparative study of two epiluminescent imaging methods suitable for the localization of a UCNP-labelled pathology site buried in highly scattering biological tissue modelled by an optical tissue phantom. The lateral resolution exhibited in scanning imaging by an illumination-collection fibre-optic probe appeared to be almost 1.73 times better than that shown by the wide-field CCD commonly used in diffuse optical tomography systems. We attribute this improved lateral resolution to the enhanced angular selectivity of the illumination-collection regime and to the nonlinear dependence of the UCNP luminescence on the excitation intensity.

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KW - Diffuse optical tomography

KW - Fibre-optic probe

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Enhanced spatial resolution in optical imaging of biotissues labelled with upconversion nanoparticles using a fibre-optic probe scanning technique

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