Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering

Katja Jouhikainen, Laura Lindgren, Tuula Jokelainen, Raimo Hiltunen, Teemu Teeri, Kirsi-Marja Oksman-Caldentey

    Research output: Contribution to journalArticleScientificpeer-review

    153 Citations (Scopus)


    In order to test the possibility of enhancing the production of pharmaceutically valuable scopolamine in transgenic cultures, the 35S-h6h transgene that codes for the enzyme hyoscyamine-6β-hydroxylase (EC was introduced into Hyoscyamus muticus L. strain Cairo (Egyptian henbane). This plant was chosen for its capability to produce very high amounts of tropane alkaloids (up to 6% of the dry weight in the leaves of mature plant). To our knowledge, this is the first time such a large population of transgenic cultures has been studied at the morphological, chemical and genetic levels. A great variation was observed in the tropane alkaloid production among the 43 positive transformants. The best clone (KB7) produced 17 mg/l scopolamine, which is over 100 times more than the control clones. However, conversion of hyoscyamine to scopolamine was still incomplete. The expression of h6h was found to be proportional to the scopolamine production, and was the main reason behind the variation in the scopolamine/hyoscyamine ratio in the hairy-root clones. These results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems.
    Original languageEnglish
    Pages (from-to)545-551
    Number of pages7
    Issue number4
    Publication statusPublished - 1999
    MoE publication typeA1 Journal article-refereed


    • Genetic engineering
    • Hairy root
    • Hyoscyamus muticus
    • Scopolamine
    • Tropane alkaloids


    Dive into the research topics of 'Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering'. Together they form a unique fingerprint.

    Cite this