Enhancement of upconversion deep-tissue imaging using optical clearing

A. P. Popov, E. V. Khaydukov, A. V. Bykov, V. A. Semchishen, V. V. Tuchin

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

5 Citations (Scopus)

Abstract

We report on the deep-tissue imaging using novel upconversion nanoparticles (UCNPs) β-NaYF4:Yb3+,Tm3+ (excitation wavelength: 975 nm, detection wavelength: 800 nm) and glycerol as an optical clearing agent to enhance imaging from under 6-mm-thick porcine muscle tissue samples. We show that improvement of luminescent label visualization is caused by transforming of the diffuse label-emitted light into the direct component. This results in the increase in visibility (ratio of the sum and difference of the maximal and minimal intensity) of the label and increase in maximal signal intensity thus making the combination of the phosphors and optical clearing promising for precise detection of tissue-embedded labelled inhomogeneities.

Original languageEnglish
Title of host publicationNovel Biophotonics Techniques and Applications III
EditorsArjen Amelink, I. Alex Vitkin
PublisherInternational Society for Optics and Photonics SPIE
ISBN (Print)978-1-6284-1705-0
DOIs
Publication statusPublished - 1 Jan 2015
MoE publication typeA4 Article in a conference publication
EventNovel Biophotonics Techniques and Applications III - Munich, Germany
Duration: 24 Jun 201525 Jun 2015

Publication series

SeriesProgress in Biomedical Optics and Imaging
Number58
Volume16
ISSN1605-7422
SeriesProceedings of SPIE
Volume9540
ISSN0277-786X

Conference

ConferenceNovel Biophotonics Techniques and Applications III
Country/TerritoryGermany
CityMunich
Period24/06/1525/06/15

Keywords

  • biotissue
  • deep-tissue imaging
  • luminescence
  • optical clearing
  • Upconversion

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