Enhancing fungal production of galactaric acid

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Galactaric (mucic) acid is a symmetrical six carbon diacid which can be produced by oxidation of galactose with nitric acid, electrolytic oxidation of d-galacturonate or microbial conversion of d-galacturonate. Both salts and the free acid of galactarate have relatively low solubility, which may create challenges for a microbial host. Galactaric acid was most soluble at pH values around 4.7 in the presence of ammonium or sodium ions and less soluble in the presence of potassium ions. Solubility increased with increasing temperature. Production of galactaric acid by Trichoderma reesei D-161646 was dependent on temperature, pH and medium composition, being best at pH 4 and 35 °C. Up to 20 g L-1 galactaric acid were produced from d-galacturonate using a fed-batch strategy with lactose as co-substrate and both ammonium and yeast extract as nitrogen sources. Crystals of galactaric acid were observed to form in the broth of some fermentations.
Original languageEnglish
Pages (from-to)4033-4040
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume101
Issue number10
DOIs
Publication statusPublished - 1 May 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Ammonium Compounds
Solubility
Ions
Nitric Acid
Trichoderma
Temperature
Lactose
Galactose
Fermentation
galactaric acid
Potassium
Nitrogen
Carbon
Salts
Yeasts
Sodium
Acids

Keywords

  • d-galacturonate
  • galactaric acid
  • mucic acid
  • solubility
  • Trichoderma reesei

Cite this

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abstract = "Galactaric (mucic) acid is a symmetrical six carbon diacid which can be produced by oxidation of galactose with nitric acid, electrolytic oxidation of d-galacturonate or microbial conversion of d-galacturonate. Both salts and the free acid of galactarate have relatively low solubility, which may create challenges for a microbial host. Galactaric acid was most soluble at pH values around 4.7 in the presence of ammonium or sodium ions and less soluble in the presence of potassium ions. Solubility increased with increasing temperature. Production of galactaric acid by Trichoderma reesei D-161646 was dependent on temperature, pH and medium composition, being best at pH 4 and 35 °C. Up to 20 g L-1 galactaric acid were produced from d-galacturonate using a fed-batch strategy with lactose as co-substrate and both ammonium and yeast extract as nitrogen sources. Crystals of galactaric acid were observed to form in the broth of some fermentations.",
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Enhancing fungal production of galactaric acid. / Barth, Dorothee; Wiebe, Marilyn G.

In: Applied Microbiology and Biotechnology, Vol. 101, No. 10, 01.05.2017, p. 4033-4040.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Enhancing fungal production of galactaric acid

AU - Barth, Dorothee

AU - Wiebe, Marilyn G.

PY - 2017/5/1

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AB - Galactaric (mucic) acid is a symmetrical six carbon diacid which can be produced by oxidation of galactose with nitric acid, electrolytic oxidation of d-galacturonate or microbial conversion of d-galacturonate. Both salts and the free acid of galactarate have relatively low solubility, which may create challenges for a microbial host. Galactaric acid was most soluble at pH values around 4.7 in the presence of ammonium or sodium ions and less soluble in the presence of potassium ions. Solubility increased with increasing temperature. Production of galactaric acid by Trichoderma reesei D-161646 was dependent on temperature, pH and medium composition, being best at pH 4 and 35 °C. Up to 20 g L-1 galactaric acid were produced from d-galacturonate using a fed-batch strategy with lactose as co-substrate and both ammonium and yeast extract as nitrogen sources. Crystals of galactaric acid were observed to form in the broth of some fermentations.

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